214c Ag Hall, Oklahoma State University, Stillwater, OK 74078-6021, USA.
J Biosci Bioeng. 2010 May;109(5):492-8. doi: 10.1016/j.jbiosc.2009.10.022. Epub 2009 Nov 18.
Fermentation of syngas offers several advantages compared to chemical catalysts such as higher specificity of biocatalysts, lower energy costs, and higher carbon efficiency. Scale-up of syngas fermentation from a bench scale to a pilot scale fermentor is a critical step leading to commercialization. The primary objective of this research was to install and commission a pilot scale fermentor, and subsequently scale-up the Clostridium strain P11 fermentation from a 7.5-L fermentor to a pilot scale 100-L fermentor. Initial preparation and fermentations were conducted in strictly anaerobic conditions. The fermentation system was maintained in a batch mode with continuous syngas supply. The effect of anaerobic fermentation in a pilot scale fermentor was evaluated. In addition, the impact of improving the syngas mass transfer coefficient on the utilization and product formation was studied. Results indicate a six fold improvement in ethanol concentration compared to serum bottle fermentation, and formation of other compounds such as isopropyl alcohol, acetic acid and butanol, which are of commercial importance.
与化学催化剂相比，合成气发酵具有几个优势，例如生物催化剂具有更高的特异性、更低的能源成本和更高的碳效率。将合成气发酵从实验室规模放大到中试规模发酵罐是实现商业化的关键步骤。本研究的主要目的是安装和调试中试规模发酵罐，随后将 Clostridium 菌株 P11 的发酵从 7.5-L 发酵罐放大到 100-L 中试规模发酵罐。最初的准备和发酵是在严格的厌氧条件下进行的。发酵系统以分批模式运行，同时连续供应合成气。评估了中试规模发酵罐中厌氧发酵的效果。此外，还研究了提高合成气传质系数对利用和产物形成的影响。结果表明，与血清瓶发酵相比，乙醇浓度提高了六倍，并且形成了其他具有商业重要性的化合物，如异丙醇、乙酸和正丁醇。
