通过 T-DNA 在水稻基因组中的整合诱导染色体倒位。

Induction of chromosomal inversion by integration of T-DNA in the rice genome.

机构信息

State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

J Genet Genomics. 2010 Mar;37(3):189-96. doi: 10.1016/S1673-8527(09)60037-0.

PMID:20347828

Abstract

Transfer DNA (T-DNA) of Agrobacterium tumefaciens integration in the plant genome may lead to rearrangements of host plant chromosomal fragments, including inversions. However, there is very little information concerning the inversion. The present study reports a transgenic rice line selected from a T-DNA tagged population, which displays a semi-dwarf phenotype. Molecular analysis of this mutant indicated an insertion of two tandem copies of T-DNA into a locus on the rice genome in a head to tail mode. This insertion of T-DNA resulted in the inversion of a 4.9 Mb chromosomal segment. Results of sequence analysis suggest that the chromosomal inversion resulted from the insertion of T-DNA with the help of sequence microhomology between insertion region of T-DNA and target sequence of the host plant.

摘要

根癌农杆菌的转移 DNA（T-DNA）整合到植物基因组中可能导致宿主植物染色体片段的重排，包括倒位。然而，关于倒位的信息非常少。本研究报告了从 T-DNA 标记群体中选择的一个转基因水稻品系，该品系表现出半矮秆表型。对该突变体的分子分析表明，两个串联的 T-DNA 拷贝以头尾模式插入到水稻基因组上的一个基因座中。这种 T-DNA 的插入导致了 4.9Mb 染色体片段的倒位。序列分析的结果表明，染色体倒位是由于 T-DNA 的插入，以及 T-DNA 插入区和宿主植物靶序列之间的序列微同源性的帮助下产生的。