Atzei Paola, Yang Fan, Collery Ross, Kennedy Breandan N, Moynagh Paul N
Institute of Immunology, National University of Ireland Maynooth, Maynooth, Co. Kildare, Ireland.
Gene Expr Patterns. 2010 Jun;10(4-5):199-206. doi: 10.1016/j.gep.2010.03.003. Epub 2010 Mar 27.
The immune system of teleost zebrafish (Danio rerio) shows high similarity to mammalian counterparts sharing many innate immune components including Toll-Like Receptors (TLRs), cytokines, chemokines and complement molecules. As in mammals, zebrafish also contains the transcription factor NF-kappaB that plays dualist roles in innate immunity and early development. Indeed NF-kappaB members are expressed in different temporal patterns during the early stages of zebrafish embryogenesis indicating that each molecule is involved in specific developmental events. In the present study we employ zebrafish as a model to characterise the expression pattern and role of a novel NF-kappaB regulator, termed Cactin, in early development. Cactin was first characterised in Drosophila as a new member of the Rel pathway that could affect the generation of dorsal-ventral polarity. To explore the potential developmental role of Cactin in zebrafish, we initially investigated its expression pattern and functional role during early embryonic developmental stages. We detect Cactin expression at all stages of early development and knockdown of Cactin by specific morpholino antisense oligonucleotides causes developmental abnormalities manifested by an overall dysmorphic cellular organisation. These results indicate that Cactin has been highly conserved during evolution and plays a key role in early embryonic development.
硬骨鱼斑马鱼（Danio rerio）的免疫系统与哺乳动物的免疫系统高度相似，共享许多先天免疫成分，包括Toll样受体（TLR）、细胞因子、趋化因子和补体分子。与哺乳动物一样，斑马鱼也含有转录因子NF-κB，它在先天免疫和早期发育中发挥双重作用。事实上，NF-κB成员在斑马鱼胚胎发育早期以不同的时间模式表达，这表明每个分子都参与特定的发育事件。在本研究中，我们以斑马鱼为模型，来表征一种名为Cactin的新型NF-κB调节因子在早期发育中的表达模式和作用。Cactin最初在果蝇中被表征为Rel途径的一个新成员，它可以影响背腹极性的产生。为了探索Cactin在斑马鱼中的潜在发育作用，我们首先研究了它在早期胚胎发育阶段的表达模式和功能作用。我们在早期发育的各个阶段都检测到了Cactin的表达，通过特定的吗啉代反义寡核苷酸敲低Cactin会导致发育异常，表现为整体细胞组织畸形。这些结果表明，Cactin在进化过程中高度保守，在早期胚胎发育中起关键作用。
