Dowling L A, Schleehauf S M
Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia.
Res Vet Sci. 1991 Mar;50(2):157-61. doi: 10.1016/0034-5288(91)90099-a.
A total of 23 (15.3 per cent) of 150 cattle infected with Mycobacterium bovis and which had never been tuberculin tested showed specific antibody responses to M bovis. Their sera may be important keys to the identification of unique M bovis antigens for use in specific serodiagnostic tests. Assessment of specific and non-specific responses was done by screening sera in six indirect anti-IgG enzyme-linked immunosorbent assays using whole cell sonicates of M bovis and five members of the Mycobacterium avium-intracellulare-scrofulaceum complex as respective antigens. Sera from 16 infected cattle that had been tuberculin tested positive and nine uninfected cattle (never tuberculin tested) were also assayed for specific and non-specific responses. Three other findings emerged. First, 43 of the 150 infected animals (28.7 per cent) showed no antibody responses to any of the mycobacterial antigens used. Secondly, the cattle showing the highest antibody levels were associated with the greatest cross reactivity. Lastly, the results indicated that tuberculin injections may increase antibody responses to shared, rather than specific, M bovis antigens in infected cattle.
150头感染牛分枝杆菌且从未进行过结核菌素检测的牛中，共有23头（15.3%）对牛分枝杆菌呈现特异性抗体反应。它们的血清可能是鉴定用于特定血清诊断检测的独特牛分枝杆菌抗原的重要关键。通过在六种间接抗IgG酶联免疫吸附试验中筛选血清来评估特异性和非特异性反应，分别使用牛分枝杆菌的全细胞超声裂解物以及鸟分枝杆菌-胞内分枝杆菌-瘰疬分枝杆菌复合体的五个成员作为抗原。还对16头结核菌素检测呈阳性的感染牛和9头未感染牛（从未进行过结核菌素检测）的血清进行了特异性和非特异性反应检测。还出现了其他三个发现。第一，150头感染动物中有43头（28.7%）对所使用的任何分枝杆菌抗原均未呈现抗体反应。第二，抗体水平最高的牛与最大的交叉反应性相关。最后，结果表明结核菌素注射可能会增加感染牛对共享的而非特定的牛分枝杆菌抗原的抗体反应。
