蜜蜂（Apis mellifera）5-HT1A 受体的特性及血清素在趋光行为中的作用。
Characterization of the 5-HT1A receptor of the honeybee (Apis mellifera) and involvement of serotonin in phototactic behavior.
机构信息
Institute of Biochemistry and Biology, Animal Physiology, University of Potsdam, Potsdam, Germany.
出版信息
Cell Mol Life Sci. 2010 Jul;67(14):2467-79. doi: 10.1007/s00018-010-0350-6. Epub 2010 Mar 28.
Abstract
Serotonin plays a key role in modulating various physiological and behavioral processes in both protostomes and deuterostomes. The vast majority of serotonin receptors belong to the superfamily of G-protein-coupled receptors. We report the cloning of a cDNA from the honeybee (Am5-ht1A) sharing high similarity with members of the 5-HT(1) receptor class. Activation of Am5-HT(1A) by serotonin inhibited the production of cAMP in a dose-dependent manner (EC(50) = 16.9 nM). Am5-HT(1A) was highly expressed in brain regions known to be involved in visual information processing. Using in vivo pharmacology, we could demonstrate that Am5-HT(1A) receptor ligands had a strong impact on the phototactic behavior of individual bees. The data presented here mark the first comprehensive study-from gene to behavior-of a 5-HT(1A) receptor in the honeybee, paving the way for the eventual elucidation of additional roles of this receptor subtype in the physiology and behavior of this social insect.
摘要
血清素在调节原口动物和后口动物的各种生理和行为过程中起着关键作用。绝大多数血清素受体属于 G 蛋白偶联受体超家族。我们从蜜蜂中克隆了一个 cDNA（Am5-ht1A），它与 5-HT（1）受体类别的成员具有高度相似性。血清素激活 Am5-HT（1A）以剂量依赖的方式抑制 cAMP 的产生（EC（50）= 16.9 nM）。Am5-HT（1A）在已知参与视觉信息处理的大脑区域中高度表达。通过体内药理学，我们可以证明 Am5-HT（1A）受体配体对个体蜜蜂的趋光行为有很强的影响。这里呈现的数据标志着对蜜蜂 5-HT（1A）受体的首次全面研究——从基因到行为——为最终阐明该受体亚型在这种社会性昆虫的生理学和行为中的其他作用铺平了道路。
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