微乳液电动色谱-电感耦合等离子体质谱联用及其在抗癌铂配合物分析中的应用。
The first example of MEEKC-ICP-MS coupling and its application for the analysis of anticancer platinum complexes.
机构信息
Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
出版信息
Electrophoresis. 2010 Apr;31(7):1144-1150. doi: 10.1002/elps.200900522.
MEEKC is a powerful electrodriven separation technique with many applications in different disciplines, including medicinal chemistry; however, up to now the coupling to highly sensitive and selective MS detectors was limited due to the ion suppressive effect of the commonly used surfactant SDS. Herein, the first example of the coupling of MEEKC to ICP-MS is presented and an MEEKC method for the separation of Pt(II) and Pt(IV) anticancer drugs and drug candidates was developed. Different compositions of microemulsions were evaluated and the data were compared with those collected with standard ultraviolet/visible (UV/vis) spectroscopy detection. The MEEKC-ICP-MS system was found to be more sensitive than MEEKC-UV/vis and the analysis of UV/vis silent compounds is now achievable. The migration behavior of the Pt(II) and Pt(IV) compounds under investigation is correlated to their different chemical structures.
MEEKC 是一种强大的电动分离技术，在包括药物化学在内的多个领域都有应用；然而，由于常用的表面活性剂 SDS 具有离子抑制作用，其与高灵敏度和选择性的 MS 检测器的耦合一直受到限制。本文首次提出了将 MEEKC 与 ICP-MS 耦合的示例，并开发了一种用于分离铂（II）和铂（IV）抗癌药物和候选药物的 MEEKC 方法。评估了不同组成的微乳液，并且将数据与使用标准紫外/可见（UV/vis）光谱检测收集的数据进行了比较。结果表明，MEEKC-ICP-MS 系统比 MEEKC-UV/vis 更灵敏，并且现在可以分析 UV/vis 静默化合物。所研究的 Pt（II）和 Pt（IV）化合物的迁移行为与其不同的化学结构有关。
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