Altria K D
Pharmaceutical Development, Glaxo Wellcome R&D, Ware, Herts., UK.
J Chromatogr A. 2000 Sep 15;892(1-2):171-86. doi: 10.1016/s0021-9673(00)00088-1.
Microemulsion electrokinetic chromatography (MEEKC) is an electrodriven separation technique. Separations are achieved using microemulsions which are nanometre-sized oil droplets suspended in aqueous buffer. The surface tension between the oil and water components is reduced by covered the oil droplet with an anionic surfactant such as sodium dodecyl sulphate and a co-surfactant such as a short-chain alcohol. This review summarises the various microemulsion types and compositions that have been used in MEEKC. The effects of key operating variables such as pH and temperature are also described. The application areas of MEEKC are also described in some detail. MEEKC has been applied to a wide range of water-soluble and insoluble both charged and neutral compounds. Examples are described which include analysis of derivatised sugars, proteins, pesticides and a wide range of pharmaceuticals. At present there are only a limited number of publications describing the use of MEEKC but it is anticipated that this number will increase rapidly in the near future as more awareness of the separation possibilities that MEEKC presents increases.
微乳液电动色谱法（MEEKC）是一种电驱动的分离技术。分离是通过使用微乳液来实现的，微乳液是悬浮在水性缓冲液中的纳米级油滴。通过用阴离子表面活性剂（如十二烷基硫酸钠）和助表面活性剂（如短链醇）覆盖油滴，可降低油相和水相成分之间的表面张力。本综述总结了在MEEKC中使用的各种微乳液类型和组成。还描述了关键操作变量（如pH值和温度）的影响。还详细介绍了MEEKC的应用领域。MEEKC已应用于广泛的水溶性和不溶性带电及中性化合物。文中列举了一些实例，包括衍生化糖类、蛋白质、农药和多种药物的分析。目前，描述MEEKC使用的出版物数量有限，但预计随着对MEEKC所呈现的分离可能性的认识不断提高，这一数量在不久的将来将迅速增加。
