Xu Jing, Sun Changkai, Ma Hui, Wang Lu, Zhang Jian, Zhang Yumei, Wu Lanxiang
Functional laboratory, Dalian Medical University, Dalian 116044, Chiha.
Zhongguo Zhong Yao Za Zhi. 2010 Jan;35(1):114-7.
To observe the effect of the extract of Ginkgo biloba (EGb761) and the components isolated from the extract named ginkgolide B (GB) against damage of glutamate in pretreatment modes so that determine their application value and approach.
Based on glutamate-induced excitotoxicity to primary cultures from neonatal Sprague-Dawley (SD) rat hippocampal neuron, our experiment utilized trypan blue, TUNEL and LDH to study the effect of EGb761 and GB on neuron in different doses pretreatment modes, as well as to compare with the NMDA receptor uncompetitive antagonist-MK-801.
EGb761 and GB can recrease cell viability, reduce apoptosis rate and decrease LDH leakage in different degree and depended on dose in certain range. The maximal protection was achieved at a concentration of 100 mg x L(-1), 100 micromol x L(-1), but inferior to MK-801 (10 micromol x L(-1)). The protective effect of GB is superior to EGb761.
Treatment with EGb761 and GB could protect the neurons against glutamate-induced injury. The maximal protection of GB was achieved by pretreatment is superior to EGb761, so its precautionanary intervention to high-risk population could have more value.
观察银杏叶提取物（EGb761）及其分离成分银杏内酯B（GB）在预处理模式下对抗谷氨酸损伤的作用，以确定其应用价值及途径。
基于谷氨酸诱导新生Sprague-Dawley（SD）大鼠海马神经元原代培养物的兴奋性毒性，本实验利用台盼蓝、TUNEL和乳酸脱氢酶（LDH）研究EGb761和GB在不同剂量预处理模式下对神经元的影响，并与NMDA受体非竞争性拮抗剂MK-801进行比较。
EGb761和GB能不同程度地提高细胞活力、降低凋亡率并减少LDH泄漏，且在一定范围内呈剂量依赖性。在浓度为100 mg·L⁻¹、100 μmol·L⁻¹时达到最大保护作用，但低于MK-801（10 μmol·L⁻¹）。GB的保护作用优于EGb761。
EGb761和GB处理可保护神经元免受谷氨酸诱导的损伤。GB预处理的最大保护作用优于EGb761，因此其对高危人群的预防性干预可能具有更大价值。
