Allender W J, Keegan J
Chemistry Section, Biological and Chemical Research Institute, Rydalmere, New South Wales, Australia.
Vet Hum Toxicol. 1991 Apr;33(2):161-5.
A method is described for the analysis of alpha-chloralose in avian tissues. The procedure involves extraction of chloralose with acetone, drying of extracts with anyhydrous magnesium sulphate, followed by derivatisation with dichlorodimethylsilane in the presence of triethylamine. The dimethylsilyl derivatives were cleaned-up using mini-columns containing Florisil and the eluates subsequently analysed by gas chromatography. Extracts were initially screened by thin-layer chromatography and the identity of derivatised extracts confirmed by gas chromatography/mass spectrometry.
描述了一种分析禽类组织中α-氯醛糖的方法。该程序包括用丙酮提取氯醛糖，用无水硫酸镁干燥提取物，然后在三乙胺存在下用二氯二甲基硅烷进行衍生化。使用含有弗罗里硅土的微型柱对二甲基硅烷基衍生物进行净化，随后通过气相色谱法对洗脱液进行分析。提取物首先通过薄层色谱法进行筛选，衍生化提取物的身份通过气相色谱/质谱法进行确认。
