Determination of sodium fluoroacetate (Compound 1080) in biological tissues.

A sensitive gas chromatographic method was developed for the determination of sodium fluoroacetate (Compound 1080 and 1080 poison) in baits and avian tissues. The procedure involves extraction of 1080 with acetone/water (8:1) followed by derivatization with pentafluorobenzyl bromide. Cleanup of the esterified extracts was carried out using minicolumns containing Florisil and the eluates were subsequently analyzed by electron capture gas chromatography. Bait samples were initially screened by thin-layer chromatography and identity of derivatized extracts was confirmed by gas chromatography/mass spectrometry.