Han Shuangyan, Li Huazhen, Jin Zi, Huang Dengfeng, Ren Changqiong, Lin Ying
School ofBioscience and Bioengineering, South China University ofTechnology, Guangzhou 510006, China.
Sheng Wu Gong Cheng Xue Bao. 2009 Dec;25(12):1784-8.
Yeast surface display involves that the exogenous protein, which was fused with the yeast outer shell cell wall protein, was genetically anchored on the yeast cell surface. It has been widely used in expression and screening of functional protein. Here, we focused on the construction of lipase-displaying systems and its application in enzymatic biosynthesis, such as fatty acid methyl esters, short-chain flavour esters and sugar esters applications, and so on.
酵母表面展示是指将与酵母外壳细胞壁蛋白融合的外源蛋白通过基因手段锚定在酵母细胞表面。它已广泛应用于功能蛋白的表达和筛选。在此,我们着重于脂肪酶展示系统的构建及其在酶促生物合成中的应用,如脂肪酸甲酯、短链风味酯和糖酯等的应用等等。