Li Hongmei, Huang Luqi, Zhou Aixiang, Li Xiaoqin, Sun Jianhui
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2009 Dec;34(24):3251-4.
To study the antiinflammatory effects of naphtha from different chemotypes of Cinnamomum camphora and natural borneol on the rat arthritis model induced by Freund's adjuvant.
The arthritis model was induced by injecting Freund's adjuvant in rat voix pedis dermis and the rats were randomly divided into seven groups: normal control group, model control group, triptergium wilfordii control group, borneol chemotype naphtha group, camphor chemotype naphtha group, isocamphane chemotype naphtha group and natural borneol group. Rats of the triptergium wilfordii control group were given orally 8.1 mg x kg(-1) triptergium wilfordii for 35 days, rats of the normal control group and model control group were given same volume water, and rats of other groups were given 80 mg x kg(-1) corresponding drug. We observed the rat common condition, weighed the rat body weight weekly, measured the degree of swelling of voix pedis every 4 days, weighed the thymus and spleen on the end of life, and measured the contents of cell factor TNF-alpha, IL-2, and IL-6 in rat blood serum.
As far as the arthrosis degree of swelling and the contents of cell factor TNF-alpha, IL-2, IL-6 were concerned, rats of model control group were higher than normal control group, and rats of other drug groups were lower than the model control group. The order of inhibition ratios of the arthrosis degree of swelling from high to low principle was isocamphane chemotype naphtha group, camphor chemotype naphtha group, borneol chemotype naphtha group and natural borneol group. All medication administration teams evidently reduced the contents of the IL-2 and IL-6, and the inhibition ratios were higher than 38%. In the case of the contents of TNF-alpha and IL-2, all groups were not evidently different. In the case of inhibition of IL-6, camphor chemotype naphtha group was better than borneol chemotype naphtha group and natural borneol group, the latter was better than isocamphane chemotype naphtha group. As far as the weight, thymus index and spleen index were concerned, all medication administration groups were not different.
The different chemotypes of C. camphora have anti-inflammatory effect on the rat arthritis model induced by Freund's adjuvant, but pharmacological activity and mechanism of action are different. The study points out the clinical curative effects of the chemotypes of the kindred medicinal plant are different, and please consider the difference of chemotype in clinical application.
研究不同化学型樟树挥发油及天然冰片对弗氏完全佐剂诱导的大鼠关节炎模型的抗炎作用。
将弗氏完全佐剂注射于大鼠足跖皮内诱导关节炎模型，大鼠随机分为7组：正常对照组、模型对照组、雷公藤多苷对照组、龙脑樟挥发油组、樟脑樟挥发油组、异龙脑樟挥发油组和天然冰片组。雷公藤多苷对照组大鼠灌胃给予雷公藤多苷8.1mg·kg-1，连续35天；正常对照组和模型对照组大鼠灌胃给予等体积蒸馏水；其余各组大鼠灌胃给予相应药物80mg·kg-1。观察大鼠一般情况，每周称体重1次，每4天测量足跖肿胀度1次，实验结束时称取胸腺和脾脏重量，检测大鼠血清中细胞因子TNF-α、IL-2、IL-6含量。
模型对照组大鼠关节肿胀度及细胞因子TNF-α、IL-2、IL-6含量高于正常对照组，各给药组大鼠上述指标低于模型对照组。异龙脑樟挥发油组、樟脑樟挥发油组、龙脑樟挥发油组、天然冰片组对关节肿胀度的抑制率由高到低依次为异龙脑樟挥发油组、樟脑樟挥发油组、龙脑樟挥发油组、天然冰片组。各给药组均明显降低IL-2、IL-6含量，抑制率均高于38%。各给药组对TNF-α、IL-2含量的抑制作用无明显差异。樟脑樟挥发油组对IL-6的抑制作用优于龙脑樟挥发油组和天然冰片组，龙脑樟挥发油组和天然冰片组优于异龙脑樟挥发油组。各给药组大鼠体重、胸腺指数和脾脏指数比较，差异无统计学意义。
不同化学型樟树挥发油对弗氏完全佐剂诱导的大鼠关节炎模型均有抗炎作用，但药理活性及作用机制不同。研究提示同属药用植物不同化学型临床疗效存在差异，临床应用时应考虑化学型差异。
