Wang Guanghui, Chen Haifeng, Wang Naili, Yao Xinsheng
Institute for Biomedical Research, Xiamen University, Xiamen 361005, China.
Zhongguo Zhong Yao Za Zhi. 2009 Dec;34(24):3286-8.
To investigate the anti-proliferative effect of Protobioside on the HepG2 cells and its mechanism.
The inhibitory effects of Protobioside on 3 kinds of human cancer cell line was measured by MTT assay. Apoptotic morphological changes was observed by Hoechst33528 staining technique. Cell cycle were analyzed using flow cytometry. The expression of cell cycle related protein and apoptosis related protein were determined using Western blotting.
Protobioside shows strong cytotoxic power on HepG2 cells and IC50 were 20 micromol x L(-1). The nucleus became pyknosis at 36 hours. The cells in G2/M phase increase in a dose-dependent and time-dependent manner. And the protein level of CyclinB1 was down-regulated, that of Bax was up-regulated and that of Bcl-2 was down-regulated.
The growth inhibition of Protobioside on HepG2 cells is highly related to cell cycle arrest at G2/M phase which was well correlated with the down-regulation of expression of Cyclin B1, and the induction of apoptosis via up-regulating of Bax and down-regulating Bcl-2 expression.
研究原七叶皂苷对HepG2细胞的抗增殖作用及其机制。
采用MTT法检测原七叶皂苷对3种人癌细胞系的抑制作用。用Hoechst33528染色技术观察凋亡形态学变化。采用流式细胞术分析细胞周期。用蛋白质免疫印迹法检测细胞周期相关蛋白和凋亡相关蛋白的表达。
原七叶皂苷对HepG2细胞具有较强的细胞毒作用，IC50为20 μmol·L⁻¹。36小时时细胞核固缩。G2/M期细胞呈剂量和时间依赖性增加。细胞周期蛋白B1（CyclinB1）蛋白水平下调，Bax蛋白水平上调，Bcl-2蛋白水平下调。
原七叶皂苷对HepG2细胞的生长抑制作用与细胞周期阻滞于G2/M期密切相关，这与细胞周期蛋白B1表达下调以及通过上调Bax和下调Bcl-2表达诱导凋亡密切相关。
