[Mechanism of hedysari polysaccharide in the apoptosis of human hepatocellular carcinoma HEP-G2 cells].

OBJECTIVE

To study the effects of Hedysari polysaccharide (HPS) on the proliferation and apoptosis of human hepatocellular carcinoma HEP-G2 cells and its mechanism.

METHODS

MTT assay, flow cytometry and fluorescence microscopy were used to the effects of HPS in anti-proliferation, cell cycle arresting and apoptosis induction. Flow cytometry was also used to detect the effects detect of HPS on protein expressions of Bcl-2 and Bax.

RESULTS

HPS could cause apparent inhibition on cell growth, induce G2/M phase arrest and apoptosis on HEP-G2 cells. HPS could down-regulate bcl-2 protein expression and up-regulate Bax protein expression.

CONCLUSION

HPS has anti-tumor effects, maybe by the way of inhibiting the cell growth, arresting the G2/M phase, inducing apoptosis, down-regulating bcl-2 and up-regulating Bax protein expression.