Li Shi-Gang, Zhang Yong-Qi
Medical College, China Three Gorges University, Yichang 443002, China.
Zhong Yao Cai. 2009 Aug;32(8):1249-51.
To study the effects of Hedysari polysaccharide (HPS) on the proliferation and apoptosis of human hepatocellular carcinoma HEP-G2 cells and its mechanism.
MTT assay, flow cytometry and fluorescence microscopy were used to the effects of HPS in anti-proliferation, cell cycle arresting and apoptosis induction. Flow cytometry was also used to detect the effects detect of HPS on protein expressions of Bcl-2 and Bax.
HPS could cause apparent inhibition on cell growth, induce G2/M phase arrest and apoptosis on HEP-G2 cells. HPS could down-regulate bcl-2 protein expression and up-regulate Bax protein expression.
HPS has anti-tumor effects, maybe by the way of inhibiting the cell growth, arresting the G2/M phase, inducing apoptosis, down-regulating bcl-2 and up-regulating Bax protein expression.
研究红芪多糖（HPS）对人肝癌HEP-G2细胞增殖和凋亡的影响及其机制。
采用MTT法、流式细胞术和荧光显微镜观察HPS的抗增殖、细胞周期阻滞及诱导凋亡作用。采用流式细胞术检测HPS对Bcl-2和Bax蛋白表达的影响。
HPS可明显抑制HEP-G2细胞生长，诱导G2/M期阻滞及凋亡。HPS可下调bcl-2蛋白表达，上调Bax蛋白表达。
HPS具有抗肿瘤作用，其机制可能是通过抑制细胞生长、阻滞G2/M期、诱导凋亡、下调bcl-2及上调Bax蛋白表达来实现的。
