Yuan Ji-li, Zhang Yue, Jiang Zhe-hao
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Jan;30(1):76-9.
To observe the effects of Fuzheng Huayu Recipe (FHR) on rat's renal interstitial fibrosis induced by mercuric chloride (HgCl2), and to explore preliminarily its mechanism of action.
Rats were randomly divided into four groups: the normal group, the model group, the FHR group and the vitamin E group, the latter two were treated respectively by FHR 4.6 g/kg and vitamin E 100 mg/kg. Rats model was established by oral administration of 8 mg/kg HgCl2 for 9 weeks. Serum creatinine (Cr) and urea nitrogen (BUN) content were tested with corresponding test kits; hydroxyproline (Hyp) content in kidney was assayed with hydrochloric acid hydrolysis; renal histologic change was observed with HE, Masson and methenamine silver (PASM) staining; and collagen type I (Col I), as well as protein expressions of fibronectin (FN) and alpha-smooth muscle actin (alpha-SMA) was determined with Western blot.
Compared with the model group, the kidney/body weight ratio, serum levels of Cr and BUN, kidney Hyp content, and severity of renal interstitial fibrosis in the two treated groups were significantly lower (P<0.05 or P<0.01), and the improvements were more significant in the FHR group than those in the vitamin E group; Col I and FN protein expression was also weaker in the two treated group (Col, P<0.05; FN, P<0.01); while the expression of alpha-SMA was lower in the FHR group (P<0.01), but it wasn't in the vitamin E group (P>0.05).
FHR could improve the HgCl2-induced renal function injury in rats, decrease extracellular matrix deposition and restrain renal interstitial fibrosis, the mechanism of action might be related with its inhibitory effect on myofibroblast activation.
观察扶正化瘀方（FHR）对氯化汞（HgCl₂）诱导的大鼠肾间质纤维化的影响，并初步探讨其作用机制。
将大鼠随机分为四组：正常组、模型组、FHR组和维生素E组，后两组分别给予FHR 4.6 g/kg和维生素E 100 mg/kg治疗。通过口服8 mg/kg HgCl₂建立大鼠模型，持续9周。用相应试剂盒检测血清肌酐（Cr）和尿素氮（BUN）含量；用盐酸水解法测定肾脏羟脯氨酸（Hyp）含量；用苏木精-伊红（HE）、Masson和六胺银（PASM）染色观察肾脏组织学变化；用蛋白质印迹法测定Ⅰ型胶原（Col Ⅰ）以及纤连蛋白（FN）和α-平滑肌肌动蛋白（α-SMA）的蛋白表达。
与模型组相比，两个治疗组的肾重/体重比值、血清Cr和BUN水平、肾脏Hyp含量以及肾间质纤维化严重程度均显著降低（P<0.05或P<0.01），且FHR组的改善比维生素E组更显著；两个治疗组的Col Ⅰ和FN蛋白表达也较弱（Col，P<0.05；FN，P<0.01）；而FHR组的α-SMA表达较低（P<0.01），但维生素E组无此现象（P>0.05）。
FHR可改善HgCl₂诱导的大鼠肾功能损伤，减少细胞外基质沉积并抑制肾间质纤维化，其作用机制可能与其对肌成纤维细胞活化的抑制作用有关。
