Tu Li-hua, Hu Ting-xing, Zhang Jian, Li Ren-hong, Dai Hong-zhong, Luo Shou-hua, Xiang Yuan-bin, Huang Li-hua
Sichuan Province Key Laboratory of Ecological Engineering, College of Forestry, Sichuan Agricultural University, Ya' an 625014, Sichuan, China.
Ying Yong Sheng Tai Xue Bao. 2009 Dec;20(12):2943-8.
From November 2007 to May 2009, a simulation test was conducted in a Pleioblastus amarus plantation in Rainy Area of West China to study the effects of nitrogen deposition on the activities of soil enzymes. Four treatments were installed, i.e., control (0 g N x m(-2) x a(-1)), low nitrogen (5 g N x m(-2) x a(-1)), medium nitrogen (15 g N x m(-2) x a(-1)), and high nitrogen (30 g N x m(-2) x a(-1)). Half year after N deposition, 0-20 cm soil samples were collected monthly, and the activities of peroxidase, polyphenol oxidase, cellulase, sucrase, urease, and acid phosphatase were determined. All test enzyme activities had apparent, seasonal variation, with the peak of cellulase, suerase, and acid phosphatase activities in spring, of urease activity in autumn, and of peroxidase and polyphenol oxidase activities in winter. Nitrogen deposition stimulated the activities of polyphenol oxidase, sucrase, urease, and acid phosphatase, inhibited cellulase activity, but had no significant effects on peroxidase activity. The test P. amurus plantation ecosystem was N-limited, and nitrogen deposition stimulated the decomposition of soil organic matter by microbe-enzyme system.
2007年11月至2009年5月，在中国西部多雨地区的苦竹林中进行了一项模拟试验，以研究氮沉降对土壤酶活性的影响。设置了四个处理，即对照（0 g N·m⁻²·a⁻¹）、低氮（5 g N·m⁻²·a⁻¹）、中氮（15 g N·m⁻²·a⁻¹）和高氮（30 g N·m⁻²·a⁻¹）。氮沉降半年后，每月采集0-20 cm土壤样品，测定过氧化物酶、多酚氧化酶、纤维素酶、蔗糖酶、脲酶和酸性磷酸酶的活性。所有测试酶活性均有明显的季节变化，纤维素酶、蔗糖酶和酸性磷酸酶活性在春季达到峰值，脲酶活性在秋季达到峰值，过氧化物酶和多酚氧化酶活性在冬季达到峰值。氮沉降刺激了多酚氧化酶、蔗糖酶、脲酶和酸性磷酸酶的活性，抑制了纤维素酶活性，但对过氧化物酶活性无显著影响。试验中的苦竹林生态系统受氮限制，氮沉降通过微生物-酶系统刺激了土壤有机质的分解。
