Liu Xingmao, Liu Hong, Ye Lingling, Li Shichong, Wu Benchuan, Wang Haitao, Xie Jing, Chen Zhaolie
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China.
Sheng Wu Gong Cheng Xue Bao. 2010 Jan;26(1):85-92.
By using the cell density, cell viability, Pro-UK activity, specific consumption rate of glucose (q(glc)), specific production rate of lactate (q(lac)), yield of lactate to glucose (Y(lac/glc)) and as the evaluation indexes, the growth and metabolism characteristics of pro-urokinase (Pro-UK) expressing CHO cells in serum-free suspension batch culture were examined and compared to those in serum-containing suspension batch culture. We observed hardly differences in growth and metabolism characteristics between the CHO cell populations grown in serum-free suspension batch culture and serum-containing suspension batch culture. The optimal mathematical model parameters for the CHO cells grown in suspension batch culture were obtained by non-linear programming of data representing the growth, substrate consumption and product formation of the CHO cells during logarithmic growth phase using MATLAB software, and the kinetic model of the cell growth and metabolism in serum-free culture were established.
以细胞密度、细胞活力、普佑克活性、葡萄糖比消耗速率（q(glc)）、乳酸比生成速率（q(lac)）、乳酸对葡萄糖的得率（Y(lac/glc)）为评价指标，考察并比较了表达普佑克（Pro-UK）的CHO细胞在无血清悬浮分批培养和含血清悬浮分批培养中的生长和代谢特性。我们观察到在无血清悬浮分批培养和含血清悬浮分批培养中生长的CHO细胞群体之间，其生长和代谢特性几乎没有差异。利用MATLAB软件对代表CHO细胞对数生长期生长、底物消耗和产物生成的数据进行非线性规划，获得了悬浮分批培养中CHO细胞的最佳数学模型参数，并建立了无血清培养中细胞生长和代谢的动力学模型。
