Zhu Wen-zeng, Ni Jin-xia, Tang Qiang, Dong Gui-rong, Li Hong-ying
Zhongguo Zhen Jiu. 2010 Jan;30(1):46-50.
To investigate the material base and underlying mechanism of the effect of cluster needling of scalp acupuncture on the neuronal plasticity in rats with focal cerebral infarction.
The model rats with acute cerebral infarction were made by blocking the middle cerebral artery with monofilament. One hundred and thirty two Wistar rats were randomly divided into 4 groups: sham-operation group (A), model group (B), point-to-point scalp acupuncture group (C) and cluster-needling of scalp acupunture group (D). Puncturing from "Baihui (GV 20)" to "Qubin (GB 7)" was used in group C. Cluster needling of scalp acupuncture was used in group D, in which needles were inserted forward and slantingly into "Baihui (GV 20)" and its left and right sides at 4 mm. In both groups, the treatment was carried out with rapid twirling reinforcing-reducing for 1 min then retaining needle for 30 min, once a day, 6 days in one course, for treating 4 courses. There was no treatment for group A and B. The change of neurological function was evaluated with Bederson score, while the expression of microtubule-associated protein 2 (MAP-2) in the ischemic penumbra was examined with immunohistochemistry (streptavidin-peroxidase method).
In comparison,with group B, the score of neurological function in group D decreased on 7th day (P<0.05), while the scors in group C and D also decreased on 14th and 28th days (both P<0.05). As compared with group C, the score of neurological function in group D obviously decreased on 28th days (P<0. 05). Comparing with group B, the expression of MAP-2 on the ischemic cortex was significantly increased in group D and C on 7th, 14th and 28th days (all P<0. 05), however, this expression in group D was higher than that in group C on 14th and 28th days (P<0. 05).
Cluster needling of scalp acupuncture can improve the neurological function of rats with focal cerebral infarction, and increase the expression of MAP-2 in the ischemic penumbra.
探讨头针丛刺对局灶性脑梗死大鼠神经可塑性影响的物质基础及潜在机制。
采用单丝阻断大脑中动脉法制备急性脑梗死模型大鼠。将132只Wistar大鼠随机分为4组：假手术组（A组）、模型组（B组）、头针点对点针刺组（C组）和头针丛刺组（D组）。C组采用从“百会（GV 20）”至“曲鬓（GB 7）”针刺。D组采用头针丛刺，将针向前斜刺进“百会（GV 20）”及其左右旁开4 mm处。两组均采用快速捻转补泻手法行针1 min，然后留针30 min，每日1次，6天为1个疗程，共治疗4个疗程。A组和B组不做处理。采用Bederson评分评估神经功能变化，采用免疫组织化学（链霉亲和素-过氧化物酶法）检测缺血半暗带微管相关蛋白2（MAP-2）的表达。
与B组比较，D组神经功能评分在第7天降低（P<0.05），C组和D组神经功能评分在第14天和第28天也降低（均P<0.05）。与C组比较，D组神经功能评分在第28天明显降低（P<0.05）。与B组比较，D组和C组缺血皮质MAP-2表达在第7天、第14天和第28天均显著增加（均P<0.05），但D组在第14天和第28天的表达高于C组（P<0.05）。
头针丛刺可改善局灶性脑梗死大鼠的神经功能，增加缺血半暗带MAP-2的表达。
