Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, People's Republic of China.
ACS Appl Mater Interfaces. 2009 Feb;1(2):319-27. doi: 10.1021/am8000456.
A series of hydrogels containing a biodegradable dextran (Dex) chain grafted with a hydrophobic poly(-caprolactone)-2-hydroxylethyl methacrylate (PCL-HEMA) chain and a thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) chain were synthesized. The molecular weight of PCL-HEMA was determined by gel permeation chromatography, and the inner morphology of the hydrogel was observed by scanning electron microscopy. The release profiles from the hydrogels were investigated using bovine serum albumen as a model drug. It was found that the release behavior could be adjusted by varying the composition of the hydrogel. In vitro cytotoxicity studies of the hydrogels showed that the copolymer Dex-PCL-HEMA/PNIPAAm exhibited low cytotoxicity. The in vivo degradation and histological studies demonstrated that the hydrogels had good biocompatibility and were promising for use as an injectable polymeric scaffold for tissue engineering applications.
合成了一系列含有可生物降解葡聚糖（Dex）链的水凝胶，该链接枝有疏水性聚（-己内酯）-2-羟乙酯基甲基丙烯酸酯（PCL-HEMA）链和温敏性聚（N-异丙基丙烯酰胺）（PNIPAAm）链。通过凝胶渗透色谱法测定了 PCL-HEMA 的分子量，并通过扫描电子显微镜观察了水凝胶的内部形态。使用牛血清白蛋白作为模型药物研究了水凝胶的释放曲线。结果发现，通过改变水凝胶的组成可以调节释放行为。水凝胶的体外细胞毒性研究表明，共聚物 Dex-PCL-HEMA/PNIPAAm 表现出低细胞毒性。体内降解和组织学研究表明，水凝胶具有良好的生物相容性，有望作为组织工程应用的可注射聚合物支架。
