Wu De-Qun, Sun Yun-Xia, Xu Xiao-Ding, Cheng Si-Xue, Zhang Xian-Zheng, Zhuo Ren-Xi
Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry,Wuhan University, Wuhan 430072, People's Republic of China.
Biomacromolecules. 2008 Apr;9(4):1155-62. doi: 10.1021/bm7010328. Epub 2008 Feb 29.
Hydrogels with pH-sensitive poly(acrylic acid) (PAAc) chains and biodegradable acryloyl-poly(-caprolactone)-2-hydroxylethyl methacrylate (AC-PCL-HEMA) chains were designed and synthesized. The morphology of hydrogel was observed by scanning electron microscopy. The degradation of the hydrogel in the presence of Pseudomonas lipase was studied. The in vitro release of bovine serum albumin from the hydrogel was investigated. Cytotoxicity study shows that the AC-PCL-HEMA/AAc copolymer exhibits good biocompatibility. Cell adhesion and migration into the hydrogel networks were evaluated by using different cell lines. The hydrogel with a lower cross-linking density and a larger pore size exhibited a better performance for cells migration.
设计并合成了具有pH敏感型聚丙烯酸（PAAc）链和可生物降解的丙烯酰基聚（ε-己内酯）-2-羟基乙基甲基丙烯酸酯（AC-PCL-HEMA）链的水凝胶。通过扫描电子显微镜观察水凝胶的形态。研究了在假单胞菌脂肪酶存在下水凝胶的降解情况。考察了水凝胶中牛血清白蛋白的体外释放。细胞毒性研究表明，AC-PCL-HEMA/AAc共聚物具有良好的生物相容性。使用不同细胞系评估细胞在水凝胶网络中的黏附与迁移。交联密度较低且孔径较大的水凝胶对细胞迁移表现出更好的性能。
