Department of Plant Physiology and Ecology, Instituto de Recursos Naturales, Centro de Ciencias Medioambientales, CSIC, Serrano, Madrid, Spain.
Plant Biotechnol J. 2010 Dec;8(9):954-65. doi: 10.1111/j.1467-7652.2010.00519.x.
Several recent studies have demonstrated that the expression of a cyanobacterial flavodoxin in plants can provide tolerance to a wide range of environmental stresses. Indeed, this strategy has been proposed as a potentially powerful biotechnological tool to generate multiple-tolerant crops. To determine whether flavodoxin expression specifically increased tolerance to salt stress and whether it might also preserve legume nitrogen fixation under saline conditions, the flavodoxin gene was introduced into the model legume Medicago truncatula. Expression of flavodoxin did not confer saline tolerance to the whole plant, although the sensitive nitrogen-fixing activity was maintained under salt stress in flavodoxin-expressing plants. Our results indicate that flavodoxin induced small but significant changes in the enzymatic activities involved in the nodule redox balance that might be responsible for the positive effect on nitrogen fixation. Expression of flavodoxin can be regarded as a potential tool to improve legume symbiotic performance under salt stress, and possibly other environmental stresses.
几项最近的研究表明，在植物中表达蓝藻黄素可以提供对广泛环境胁迫的耐受性。事实上，这种策略已被提议作为一种潜在的强大生物技术工具，以产生多种耐受作物。为了确定黄素蛋白表达是否特异性地增加了对盐胁迫的耐受性，并且它是否也可以在盐胁迫条件下保存豆科植物的固氮作用，黄素蛋白基因被引入模式豆科植物苜蓿。黄素蛋白的表达并没有赋予整个植物耐盐性，尽管在表达黄素蛋白的植物中，敏感的固氮活性在盐胁迫下得以维持。我们的结果表明，黄素蛋白诱导了与根瘤氧化还原平衡相关的酶活性的微小但显著的变化，这可能是对固氮作用产生积极影响的原因。黄素蛋白的表达可以被视为一种提高豆科植物共生性能在盐胁迫下的潜在工具，并且可能在其他环境胁迫下也是如此。
