China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Chinese MoST International Cooperation Base of Science and Technology (Genetic Engineering), Chinese MoE Key Laboratory of Molecular Biophysics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
J Sci Food Agric. 2010 Jan 15;90(1):106-11. doi: 10.1002/jsfa.3790.
The transfer of transgenes from 'model' wheat genotypes into elite wheat cultivars using conventional plant breeding is an alternative strategy for improving the dough quality of wheat. Thus a cross was made between a popular Chinese elite wheat cultivar of the Yangzi River down-central area that expresses high-molecular-weight glutenin subunit (HMW-GS): 1Bx7 + 1By8 plus 1Dx2 + 1Dy12, and a model transgenic wheat line B102-1-2 which over-expresses HMW-GS 1Ax1 in an L88-31 genetic background that includes HMW-GS 1Bx17 + By18.
F(1) to F(6) generations of crosses between B102-1-2 (paternal) and Emai (maternal) were analysed for their HMW-GS compositions, allowing the selection of pure F(6) lines over-expressing HMS-GS 1Ax1 in the presence of the endogenous HMW-GS: 1Bx7 + 1By8 with 1Dx2 + 1Dy12, and 1Bx17 + 1By18 with 1Dx2 + 1Dy12. Analysis of the F(6) lines showed changes in the extensograph parameters, with increases in peak area, resistance to extension, and extensibility and peak resistance to extension, similar to those observed in the transgenic paternal line B102-1-2.
This work showed that the expression levels of the 1Ax1 transgene and the effects on dough properties were similar in the transgenic parental line B102-1-2 and the F(6) progeny generated from a cross with an elite Chinese cultivar Emai. The feasibility of using transgenic lines expressing HMW-GS subunits in conventional breeding programmes was demonstrated.
通过传统植物育种将转基因从“模式”小麦基因型转移到优质小麦品种中，是改善小麦面团品质的一种替代策略。因此，在长江中下游地区一个受欢迎的中国优质小麦品种与一个在 L88-31 遗传背景下表达高分子量谷蛋白亚基（HMW-GS）1Ax1 的模式转基因小麦品系 B102-1-2 之间进行了杂交，该品系包含 HMW-GS 1Bx17 + By18。
对 B102-1-2（父本）和 Emai（母本）杂交的 F1 至 F6 代进行了 HMW-GS 组成分析，允许在存在内源性 HMW-GS：1Bx7 + 1By8 与 1Dx2 + 1Dy12 和 1Bx17 + 1By18 与 1Dx2 + 1Dy12 的情况下，选择纯 F6 系过度表达 HMS-GS 1Ax1。对 F6 系的分析表明，拉伸仪参数发生了变化，峰面积、延伸阻力、延展性和延伸峰值阻力增加，与转基因父本系 B102-1-2 观察到的相似。
这项工作表明，在转基因亲本系 B102-1-2 和与中国优质品种 Emai 杂交产生的 F6 后代中，1Ax1 转基因的表达水平及其对面团特性的影响相似。证明了在常规育种计划中使用表达 HMW-GS 亚基的转基因系的可行性。
