Immunanalysis Corporation, 829 Towne Center Drive, Pomona, CA 91767, USA.
Drug Test Anal. 2009 May;1(5):234-9. doi: 10.1002/dta.40.
A quantitative analytical procedure for the determination of Delta(9)-tetrahydrocannabinol (THC) in hair has been developed and validated using liquid chromatography with tandem mass spectral detection (LC-MS/MS). Specimens that were determined as containing cannabinoids following immunoassay testing were quantified using solid-phase extraction followed by liquid chromatographic separation and tandem mass spectral detection in positive electrospray ionization mode. For confirmation, two transitions were monitored and one ratio determined. Samples being reported as positive were required to have both transitions present, the ratio of quantifying transition to qualifying transition being within 20% of that determined from known calibration standards. The limit of quantitation and the limit of detection was 10 pg/mg. The percentage recovery of the THC from hair at 20 pg/mg was 56% and a matrix effect of the hair showed an ion suppression percentage of -51%. The immunochemical screening method was performed following a rapid aqueous extraction, requiring only 10 mg of hair; the confirmatory procedure required 20 mg of hair. The methods were applied to proficiency specimens from the Society of Hair Testing, which had been received in August 2008.
已经建立并验证了一种使用液相色谱-串联质谱检测（LC-MS/MS）定量分析毛发中Δ9-四氢大麻酚（THC）的方法。采用免疫分析法检测出含有大麻素的样本，用固相萃取法进行定量，然后进行液相色谱分离和正电喷雾电离模式下的串联质谱检测。为了确认，监测了两个转换，并确定了一个比值。报告为阳性的样本必须同时存在两个转换，定量转换与定性转换的比值应在已知校准标准确定的 20%以内。定量下限和检测下限为 10 pg/mg。在 20 pg/mg 时，从毛发中回收的 THC 百分比为 56%，毛发的基质效应显示离子抑制百分比为-51%。免疫化学筛选方法在快速水提取后进行，仅需 10 mg 的毛发；确证程序需要 20 mg 的毛发。该方法应用于 2008 年 8 月收到的毛发测试协会的能力验证样本。
