Department of Anatomy, School of Medicine, Chungnam National University, Taejon 301-747, Korea.
Z Naturforsch C J Biosci. 2010 Jan-Feb;65(1-2):153-6. doi: 10.1515/znc-2010-1-224.
Using silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human granulocyte macrophage colony-stimulating factor (hGM-CSF), which gets its name from the Bm5-hGM-CSF cell in which the glycoprotein of the hGM-CSF is secreted in the cell culture supernatant (CCS). It was demonstrated that secreted hGM-CSF had in vivo biological activity and the white blood cell (WBC) value increased two times that of the control. We expect to produce useful human recombinant glycoproteins from silkworm cultured cells for a low price and a large quantity.
利用家蚕 Bombyx mori Bm5 细胞，我们建立了一个稳定表达人粒细胞-巨噬细胞集落刺激因子（hGM-CSF）的细胞系，该细胞系因在细胞培养上清液（CCS）中分泌 hGM-CSF 糖蛋白的 Bm5-hGM-CSF 细胞而得名。研究表明，分泌的 hGM-CSF 具有体内生物活性，白细胞（WBC）值增加到对照组的两倍。我们期望从蚕培养细胞中以较低的价格和大量生产出有用的人重组糖蛋白。
