Kim Kyo-Il, Lee Jae-Chan, Robards Kevin, Choi Seong-Ho
Department of Chemistry, BK 21 NanoBiosensor Research Team, Hannam University, Daejeon 305-811, Republic of Korea.
J Nanosci Nanotechnol. 2010 Jun;10(6):3790-8. doi: 10.1166/jnn.2010.1982.
Tyrosinase-immobilized biosensor was fabricated based on PAAc-g-MWNT and PMAn-g-MWNT, respectively. The poly(acrylic acid)-grafted multi-wall carbon nanotubes, PAAc-g-MWNT, and poly(maleic anhydride)-grafted multi-wall carbon nanotube, PMAn-g-MWNT, were prepared by radiation-induced graft polymerization of acrylic acid (AAc) and maleic anhydride (MAn) on the surface of MWNT. The biosensor was prepared on ITO glass electrode by coating of chitosan solution with tyrosinase-immobilized PAAc-g-MWNT and PMAn-g-MWNT, respectively. The sensing ranges of the tyrosinase-immobilized biosensor based on PAAc-g-MWNT and PMAn were in the range of 0.2-0.9 mM concentration and in the range of 0.1-0.5 mM for phenol in phosphate buffer solution, respectively. Optimal pH and temperature conditions for sensing various phenolic compounds with tyrosinase-immobilized biosensor were determined. Total phenolic content for three commercial red wines on tyrosinase-immobilized biosensor were also determined.
分别基于聚丙烯酸接枝多壁碳纳米管（PAAc-g-MWNT）和聚马来酸酐接枝多壁碳纳米管（PMAn-g-MWNT）制备了酪氨酸酶固定化生物传感器。通过在多壁碳纳米管表面进行丙烯酸（AAc）和马来酸酐（MAn）的辐射诱导接枝聚合反应，制备了聚丙烯酸接枝多壁碳纳米管（PAAc-g-MWNT）和聚马来酸酐接枝多壁碳纳米管（PMAn-g-MWNT）。通过分别用固定有酪氨酸酶的PAAc-g-MWNT和PMAn-g-MWNT包覆壳聚糖溶液，在氧化铟锡（ITO）玻璃电极上制备生物传感器。基于PAAc-g-MWNT和PMAn的酪氨酸酶固定化生物传感器在磷酸盐缓冲溶液中对苯酚的传感范围分别为0.2 - 0.9 mM浓度和0.1 - 0.5 mM。确定了用酪氨酸酶固定化生物传感器检测各种酚类化合物的最佳pH和温度条件。还测定了三种市售红酒在酪氨酸酶固定化生物传感器上的总酚含量。
