Yamaguchi K, Ohnishi T
Histochemistry. 1977 Dec 7;54(3):191-9. doi: 10.1007/BF00492241.
Histochemical investigation shows that ATPase activity is located intensively on the surface of cell contacts in hepatoma cells grown in confluent monolayer culture. Dibutyryl cyclic AMP and theophylline-treated hepatoma cells which exhibit contact-inhibited growth show the absence of localization of intense ATPase activity at cell-cell contacts. However, after removal of these additives the activity fully recovers to the intense level of control cells, when the release of cells from contact inhibition of growth occurs. Cultured hepatic parenchymal cells in monolayer have little or no ATPase activity at their surface immediately after contacts are established, and again after growth to a confluent state. In a different type of hepatoma cell which is less malignant and forms a small tissue mass or island, cell surface ATPase activity at cell-cell contacts in an island is very weak or scarcely detected even when cells are not treated with dibutyryl cyclic AMP and theophylline.
组织化学研究表明，在汇合单层培养中生长的肝癌细胞中，ATP酶活性密集地位于细胞接触表面。经二丁酰环磷酸腺苷和茶碱处理的肝癌细胞呈现接触抑制生长，在细胞 - 细胞接触处未显示出强烈的ATP酶活性定位。然而，去除这些添加剂后，当细胞从生长的接触抑制中释放时，活性完全恢复到对照细胞的强烈水平。单层培养的肝实质细胞在建立接触后立即以及再次生长至汇合状态后，其表面几乎没有或没有ATP酶活性。在另一种恶性程度较低且形成小组织块或岛状的肝癌细胞类型中，即使细胞未用二丁酰环磷酸腺苷和茶碱处理，岛状结构中细胞 - 细胞接触处的细胞表面ATP酶活性也非常弱或几乎检测不到。
