ACS Appl Mater Interfaces. 2009 Mar;1(3):543-8. doi: 10.1021/am800264h.
Cell adhesion is an important process in several biological phenomena. To investigate the formation and organization of focal adhesions, we developed a patterning approach based on electron beam lithography. Nanodots (radius <1230 nm) and nanorings (inner radius <320 nm) of fibronectin (FN) were patterned on a K-Casein background. Intracellular vinculin immunofluorescence mirrored the FN nanopatterns. Atomic force microscopy showed that FN nanodots and nanorings organize the immediate cytoskeleton into straight fibrils and diverging fibril bundles, respectively. Our results suggest that a minimum of approximately 40 FN molecules is required for a cell to form a focal adhesion.
细胞黏附是几种生物现象中的一个重要过程。为了研究黏着斑的形成和组织，我们开发了一种基于电子束光刻的图案化方法。在 K-酪蛋白背景上对纤连蛋白（FN）的纳米点（半径 <1230nm）和纳米环（内半径 <320nm）进行图案化。细胞内 vinculin 免疫荧光反映了 FN 的纳米图案。原子力显微镜显示，FN 纳米点和纳米环分别将即刻细胞骨架组织成直纤维和发散纤维束。我们的结果表明，细胞形成黏着斑至少需要大约 40 个 FN 分子。
