Department of Metal and Materials Engineering, Kangnung National University 120, Kangnung Daehangno, Kangnung 210-702, Korea.
ACS Nano. 2010 Apr 27;4(4):2070-6. doi: 10.1021/nn901312f.
Optical interferometry of a thin film array of titanium dioxide (TiO2) nanotubes allows the label-free sensing of rabbit immunoglobulin G (IgG). A protein A capture probe is used, which is immobilized on the inner pore walls of the nanotubes by electrostatic adsorption. Control experiments using IgG from chicken (which does not bind to protein A) confirms the specificity of the protein A-modified TiO2 nanotube array sensor. The aqueous stability of the TiO2 nanotube array was examined and compared with porous silica (SiO2), a more extensively studied thin film optical biosensor. The TiO2 nanotube array is stable in the pH range 2 to 12, whereas the porous SiO2 sensor displays significant degradation at pH > 8.
采用二氧化钛（TiO2）纳米管薄膜阵列的光学干涉法，可以实现对兔免疫球蛋白 G（IgG）的无标记传感。使用一种蛋白质 A 捕获探针，通过静电吸附固定在纳米管的内孔壁上。使用来自鸡的 IgG（其不与蛋白质 A 结合）的对照实验证实了蛋白质 A 修饰的 TiO2 纳米管阵列传感器的特异性。还检查并比较了 TiO2 纳米管阵列与多孔氧化硅（SiO2）的水稳定性，后者是一种更广泛研究的薄膜光学生物传感器。TiO2 纳米管阵列在 pH 值 2 至 12 的范围内稳定，而多孔 SiO2 传感器在 pH 值 > 8 时会显著降解。
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