He Jie-Long, Wang Da-Shin, Fan Shih-Kang
Department of Mechanical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Micromachines (Basel). 2016 Feb 15;7(2):29. doi: 10.3390/mi7020029.
Optical detection has long been the most popular technique in immunosensing. Recent developments in the synthesis of luminescent probes and the fabrication of novel nanostructures enable more sensitive and efficient optical detection, which can be miniaturized and integrated with microfluidics to realize compact lab-on-a-chip immunosensors. These immunosensors are portable, economical and automated, but their sensitivity is not compromised. This review focuses on the incorporation and implementation of optical detection and microfluidics in immunosensors; it introduces the working principles of each optical detection technique and how it can be exploited in immunosensing. The recent progress in various opto-microfluidic immunosensor designs is described. Instead of being comprehensive to include all opto-microfluidic platforms, the report centers on the designs that are promising for point-of-care immunosensing diagnostics, in which ease of use, stability and cost-effective fabrication are emphasized.
光学检测长期以来一直是免疫传感中最受欢迎的技术。发光探针合成和新型纳米结构制造方面的最新进展使得光学检测更加灵敏和高效,这种检测可以小型化并与微流体技术集成,以实现紧凑的芯片实验室免疫传感器。这些免疫传感器便于携带、经济且自动化,但并不影响其灵敏度。本综述重点关注光学检测和微流体技术在免疫传感器中的结合与应用;介绍了每种光学检测技术的工作原理以及如何将其应用于免疫传感。文中描述了各种光微流体免疫传感器设计的最新进展。该报告并非全面涵盖所有光微流体平台,而是聚焦于有望用于即时护理免疫传感诊断的设计,其中强调了易用性、稳定性和经济高效的制造。