Wang Hui-xiang, Tan Yan
Department of Obstetrics and Gynecology, Affiliated Yuquan Hospital of Tsinghua University, Beijing 100049, China.
Zhonghua Yi Xue Za Zhi. 2010 Jan 5;90(1):61-3.
To study the expression of NASP in platinum resistance ovarian cancer cell clones (C0C1/DDP).
The proteins of C0C1 and C0C1/DDP were separated by highly sensitive two-dimensional differential gel electrophoresis (2-D DIGE) coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF-MS) to identify the proteins differentially expressed in cisplatin sensitive and cisplatin resistance cell lines.
The investigators found a group of the distinct expressed proteins between C0C1 and C0C1/DDP. Among them, NASP was one of the down-regulated proteins by 21% (Student's t-test; P = 0.01).
The identification of NASP in C0C1/DDP has laid a foundation for future in-depth researches into the mechanisms of ovarian cancer platinum resistance.
研究核自体抗原精子蛋白（NASP）在铂耐药卵巢癌细胞克隆（C0C1/DDP）中的表达。
采用高灵敏度二维差异凝胶电泳（2-D DIGE）结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分离C0C1和C0C1/DDP的蛋白质，以鉴定在顺铂敏感和顺铂耐药细胞系中差异表达的蛋白质。
研究者发现C0C1和C0C1/DDP之间存在一组差异表达的蛋白质。其中，NASP是下调21%的蛋白质之一（学生t检验；P = 0.01）。
C0C1/DDP中NASP的鉴定为今后深入研究卵巢癌铂耐药机制奠定了基础。
