Xiao Li, Shi Bing-Yi, Cai Ming, He Xiu-Yun, Xu Xiao-Guang, Han Yong, Zhou Wen-Qiang, Meng Xiao-Yun, Gao Yu
Basic Research Laboratory of Organ Transplant Center, No. 309 Hospital of Chinese People's Liberation Army, Beijing 100091, China.
Zhonghua Yi Xue Za Zhi. 2010 Jan 26;90(4):241-4.
To investigate the expression of non-classical major histocompatibility complex (MHC)-I molecule, human leucocyte antigen (HLA) G, including membrane-bound HLA-G (mHLA-G), intracellular HLA-G (iHLA-G) and soluble HLA-G (sHLA-G), in peripheral blood of surviving kidney transplantation recipients and understand the relevance between HLA-G and the function of transplanted organ, as well as the onset of acute rejection.
A longitudinal study was performed on 175 kidney transplantation recipients. Three groups were involved in this study, including acute rejection group (n = 36), function stable group (n = 139) and healthy control group (n = 30). The expression of mHLA-G1 and iHLA-G1 in the T lymphocytes of peripheral blood was detected by flow cytometry analysis and the sHLA-G5 level detected by ELISA.
The average rate of CD4(+)mHLA-G1(+), CD8(+)mHLA-G1(+), CD4(+)iHLA-G1(+), CD8(+)iHLA-G1(+) in T lymphocytes of healthy control group was 0.43% +/- 0.19%, 1.23% +/- 0.41%, 27% +/- 13% and 36% +/- 14% respectively. That of acute rejection group was 0.57% +/- 0.34%, 1.31% +/- 0.56%, 26% +/- 8% and 37% +/- 17%; that of function stable group was 0.61% +/- 0.43%, 1.39% +/- 0.47%, 26% +/- 9% and 37% +/- 17% respectively. There was no significant difference among the three groups (all P > 0.05). The average of sHLA-G5 levels in plasma of control group was (25 +/- 14) ng/ml, acute rejection group (24 +/- 15) ng/ml (pre-operative) and (34 +/- 21) ng/ml (post-operative), function stable group (25 +/- 11) ng/ml (pre-operative) and (56 +/- 32) ng/ml (post-operative). There was no significant difference among the three groups (pre-operative, P > 0.05). The average of sHLA-G5 levels in plasma of function stable group was higher than that of acute rejection group (post-operative, P < 0.05).
There is a subset of CD4(+)HLA-G1(+) and CD8(+)HLA-G1(+)T lymphocytes with low percentage in peripheral blood of those surviving kidney transplantation recipients. The expressions of mHLA-G1 and iHLA-G1 have no relevance with the onset of acute rejection. sHLA-G5 is correlated with acute rejection in peripheral blood of surviving transplantation recipients.
研究存活肾移植受者外周血中非经典主要组织相容性复合体（MHC）-Ⅰ类分子人类白细胞抗原（HLA）-G的表达情况，包括膜结合型HLA-G（mHLA-G）、细胞内型HLA-G（iHLA-G）和可溶性HLA-G（sHLA-G），并了解HLA-G与移植器官功能以及急性排斥反应发生之间的相关性。
对175例肾移植受者进行纵向研究。本研究分为三组，包括急性排斥反应组（n = 36）、功能稳定组（n = 139）和健康对照组（n = 30）。采用流式细胞术分析检测外周血T淋巴细胞中mHLA-G1和iHLA-G1的表达，采用酶联免疫吸附测定法检测sHLA-G5水平。
健康对照组T淋巴细胞中CD4(+)mHLA-G1(+)、CD8(+)mHLA-G1(+)、CD4(+)iHLA-G1(+)、CD8(+)iHLA-G1(+)的平均比例分别为0.43%±0.19%、1.23%±0.41%、27%±13%和36%±14%。急性排斥反应组分别为0.57%±0.34%、1.31%±0.56%、26%±8%和37%±17%；功能稳定组分别为0.61%±0.43%、1.39%±0.47%、26%±9%和37%±17%。三组之间差异均无统计学意义（均P>0.05）。对照组血浆中sHLA-G5水平平均值为（25±14）ng/ml，急性排斥反应组术前为（24±15）ng/ml，术后为（34±21）ng/ml，功能稳定组术前为（25±11）ng/ml，术后为（56±32）ng/ml。三组术前差异无统计学意义（P>0.05）。功能稳定组血浆中sHLA-G5水平平均值高于急性排斥反应组（术后，P<0.05）。
存活肾移植受者外周血中存在一定比例的CD4(+)HLA-G1(+)和CD8(+)HLA-G1(+)T淋巴细胞亚群。mHLA-G1和iHLA-G1的表达与急性排斥反应的发生无关。sHLA-G5与存活移植受者外周血中的急性排斥反应相关。
