Yau J L, Van Haarst A D, Moisan M P, Fleming S, Edwards C R, Seckl J R
Department of Medicine, University of Edinburgh, Western General Hospital, United Kingdom.
Am J Physiol. 1991 May;260(5 Pt 2):F764-7. doi: 10.1152/ajprenal.1991.260.5.F764.
11 beta-Hydroxysteroid dehydrogenase (11 beta-OHSD) protects nonspecific renal mineralocorticoid receptors from exposure to circulating glucocorticoid in vivo by catalyzing the conversion of corticosterone to inactive 11-dehydrocorticosterone. Although 11 beta-OHSD bioactivity and aldosterone binding sites are found in distal tubular cells, mineralocorticoid receptor and 11 beta-OHSD immunoreactivities are not colocalized. However, there are several kidney isoforms of 11 beta-OHSD, not all of which may be immunoreactive, whereas only a single mRNA species has been described. Using in situ hybridization we found 11 beta-OHSD mRNA is highly expressed in all renal tubular epithelia in the rat. It is therefore likely that 11 beta-OHSD is colocalized with mineralocorticoid receptors in distal tubular cells.
11β-羟基类固醇脱氢酶（11β-OHSD）通过催化皮质酮转化为无活性的11-脱氢皮质酮，在体内保护非特异性肾盐皮质激素受体免受循环糖皮质激素的影响。虽然在远端肾小管细胞中发现了11β-OHSD生物活性和醛固酮结合位点，但盐皮质激素受体和11β-OHSD免疫反应性并不共定位。然而，11β-OHSD有几种肾脏同工型，并非所有同工型都具有免疫反应性，而仅描述了一种mRNA种类。通过原位杂交，我们发现11β-OHSD mRNA在大鼠所有肾小管上皮中高度表达。因此，11β-OHSD很可能在远端肾小管细胞中与盐皮质激素受体共定位。
