Yajima M, Hosoda K, Kanbayashi Y, Nakamura T, Takahashi I, Ui M
J Biochem. 1978 Jan;83(1):305-12. doi: 10.1093/oxfordjournals.jbchem.a131905.
The biological activities were studied of a new protein, islets-activating protein (IAP), purified from the culture medium of Bordetella pertussis. Rats injected intravenously with 1 microgram of purified IAP exhibited markedly enhanced insulin secretory responses to glucose, glucagon, epinephrine, and sulfonylureas over a period from 3 to 10 days after the injection. The degree and duration of the enhancement were proportional to the dose of IAP; the maximal effect induced by 1-2 microgram of IAP persisted for as long as 2 months. There was a highly significant correlation between the enhancement of insulin secretion and suppression of epinephrine hyperglycemia over a wide range of doses of IAP, indicating that suppression of epinephrine hyperglycemia resulted from hypoglycemic action of insulin secreted in response to epinephrine challenge. Additional actions of IAP were observed in mice; mice treated with higher doses of IAP showed symptoms were observed when lower doses of IAP were injected into mice. Thus, it is concluded that IAP is a protein primarily possessing a unique action to potentiate insulin secretory responses of experimental animals to nutritional and hormonal stimuli.
对从百日咳博德特氏菌培养基中纯化得到的一种新蛋白质——胰岛激活蛋白（IAP）的生物学活性进行了研究。静脉注射1微克纯化IAP的大鼠，在注射后3至10天内，对葡萄糖、胰高血糖素、肾上腺素和磺酰脲类药物的胰岛素分泌反应显著增强。增强的程度和持续时间与IAP的剂量成正比；1至2微克IAP诱导的最大效应持续长达2个月。在很宽的IAP剂量范围内，胰岛素分泌的增强与肾上腺素性高血糖的抑制之间存在高度显著的相关性，这表明肾上腺素性高血糖的抑制是由对肾上腺素刺激分泌的胰岛素的降血糖作用所致。在小鼠中观察到了IAP的其他作用；用较高剂量IAP处理的小鼠出现了症状，而向小鼠注射较低剂量IAP时也观察到了这些症状。因此，得出结论，IAP是一种主要具有独特作用的蛋白质，可增强实验动物对营养和激素刺激的胰岛素分泌反应。
