Hewlett E L, Sauer K T, Myers G A, Cowell J L, Guerrant R L
Infect Immun. 1983 Jun;40(3):1198-203. doi: 10.1128/iai.40.3.1198-1203.1983.
Exposing Chinese hamster ovary cells in culture to pertussis toxin resulted in a novel clustered growth pattern. The specificity of the response for pertussis toxin was shown by neutralization of the activity with specific anti-toxin antibody, heat lability (80 degrees C for 15 min), and absence of such activity by culture media from nontoxigenic Bordetella species. Although a lag of at least 16 h was required before clustered growth was seen, exposure to the toxin for as little as 10 min resulted in a full response 24 h later. The morphological effect appeared to be independent of the cyclic AMP-mediated cell elongation elicited by the heat-labile enterotoxin from Vibrio cholerae in that the pertussis toxin effect was seen in both the presence and absence of elongation. Although the mechanism by which this effect is mediated remains to be determined, it is already providing a useful in vitro assay for pertussis toxin.
将培养中的中国仓鼠卵巢细胞暴露于百日咳毒素会导致一种新的聚集生长模式。通过用特异性抗毒素抗体中和活性、热不稳定性（80℃ 15分钟）以及非产毒素博德特氏菌属的培养基不存在这种活性，证明了对百日咳毒素反应的特异性。尽管在观察到聚集生长之前至少需要16小时的延迟，但暴露于毒素仅10分钟就会在24小时后产生完全反应。形态学效应似乎与霍乱弧菌热不稳定肠毒素引起的环磷酸腺苷介导的细胞伸长无关，因为在有和没有伸长的情况下都能看到百日咳毒素的效应。虽然这种效应的介导机制尚待确定，但它已经为百日咳毒素提供了一种有用的体外检测方法。
