一种免疫遗传学定义的且具有免疫显性的克氏锥虫抗原。

An immunogenetically defined and immunodominant Trypanosoma cruzi antigen.

作者信息

Ramos R, Juri M, Ramos A, Hoecker G, Lavandero S, Pena P, Morello A, Repetto Y, Aguillon J C, Ferreira A

机构信息

Universidad de Chile, Ministerio de Salud, Servicio de Salud Metropolitano Sur. Santiago.

出版信息

Am J Trop Med Hyg. 1991 Mar;44(3):314-22. doi: 10.4269/ajtmh.1991.44.314.

DOI:10.4269/ajtmh.1991.44.314

PMID:2035753

Abstract

Two strains of mice, A. SW (H-2s) and A.CA (H-2f), were immunized with live trypomastigotes or epimastigotes of the Tulahuen strain of Trypanosoma cruzi or with their sonicates. By immunowestern blotting, sera from A.SW mice, but not from A.CA, recognized, in an immunodominant fashion, a 45 kDal polypeptide (Tc45) present in both epimastigotes and trypomastigotes. Since A.SW and A.CA strains are congenic for the major histocompatibility H-2 complex, recognition of Tc45 seems to be controlled by this genetic region or by gene(s) located in its immediate vicinity. Subcellular fractionation revealed that Tc45 is mainly present at the cytoplasmic compartment.

摘要

用克氏锥虫图拉韦恩株的活锥鞭毛体或无鞭毛体及其超声裂解物对两种品系的小鼠，即A.SW（H-2s）和A.CA（H-2f）进行免疫。通过免疫印迹法，A.SW小鼠的血清而非A.CA小鼠的血清以免疫显性方式识别出存在于无鞭毛体和锥鞭毛体中的一种45 kDa多肽（Tc45）。由于A.SW和A.CA品系在主要组织相容性H-2复合体上是同源的，所以对Tc45的识别似乎受该遗传区域或其紧邻区域中的基因控制。亚细胞分级分离显示，Tc45主要存在于细胞质区室中。