Tachibana H, Nagakura K, Kaneda Y
Z Parasitenkd. 1986;72(4):433-41. doi: 10.1007/BF00927887.
Two monoclonal antibodies (designated as TCF48 and TCF87 were raised against Trypanosoma cruzi, strain Tulahuen, Both antibodies reacted with all developmental forms of several different strains of Trypanosoma cruzi. The antibodies showed no detectable cross-reactivity with other species of Trypanosomatidae, so far examined. TCF48 and TCF87 were classified as immunoglobulin subclasses IgG1 and IgG2b, respectively. Apparent molecular weight of the corresponding antigen(s) to these monoclonal antibodies was 25,000 in amastigotes and epimastigotes, and 25,000 and 24,000 in trypomastigotes, as determined by the Western immunoblotting analysis. This antigen appeared to be located at the plasma membrane and the flagellum ofT. cruzi. However, no evidence supported the localization of the epitope(s) at the external surface of the live cell. Since this antigen reacted with the sera from the chronically infected mice, these monoclonal antibodies may be useful in the study of Chagas' disease.
制备了两种抗克氏锥虫图拉温株的单克隆抗体（分别命名为TCF48和TCF87）。两种抗体均与几种不同克氏锥虫菌株的所有发育形式发生反应。到目前为止，所检测的抗体与其他锥虫科物种未显示出可检测到的交叉反应性。TCF48和TCF87分别被归类为免疫球蛋白亚类IgG1和IgG2b。通过蛋白质免疫印迹分析测定，这些单克隆抗体相应抗原在无鞭毛体和上鞭毛体中的表观分子量为25000，在锥鞭毛体中为25000和24000。该抗原似乎位于克氏锥虫的质膜和鞭毛上。然而，没有证据支持表位位于活细胞外表面。由于该抗原与慢性感染小鼠的血清发生反应，这些单克隆抗体可能有助于恰加斯病的研究。
