通过胰蛋白酶处理从腹水肝癌细胞中释放糖肽和粘多糖。
Release of glycopeptides and mucopolysaccharides from ascites hepatoma cells by tryptic treatment.
作者信息
Nakada H, Yamashina I
出版信息
J Biochem. 1978 Jan;83(1):79-83. doi: 10.1093/oxfordjournals.jbchem.a131914.
Abstract
Two types of ascites hepatoma cells, AH 66 and AH 130 FN, were treated with trypsin to observe the release of complex carbohydrates constituting the plasma membranes. From AH 66 cells, mucopolysaccharide (heparan sulfate) was preferentially released. From AH 130 FN cells, N-glycosidic glycopeptides were preferentially released whereas no mucopolysaccharide (chondroitin sulfate A) was released.
摘要
用胰蛋白酶处理两种腹水肝癌细胞AH 66和AH 130 FN，以观察构成质膜的复合碳水化合物的释放情况。从AH 66细胞中，优先释放出粘多糖（硫酸乙酰肝素）。从AH 130 FN细胞中，优先释放出N-糖苷糖肽，而没有释放出粘多糖（硫酸软骨素A）。
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