Karrow Niel A, You Qiumei, McNicoll Carl, Hay Jack
Centre for the Genetic Improvement of Livestock, Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario.
Can J Vet Res. 2010 Jan;74(1):30-3.
Sheep were subjected to immune challenge with either recombinant human interleukin-6 (rhIL-6; 2.0 microg/kg; n = 5), Escherichia coli lipopolysaccharide (LPS) endotoxin (400 ng/kg; n = 7), or saline (n = 6) to determine if IL-6 activates the febrile and hypothalamic-pituitary-adrenal axis (HPAA) responses in sheep, and to compare these responses with those associated with endotoxemia. Blood was collected over time to measure plasma adrenocorticotropic hormone (ACTH) and serum cortisol concentrations as indicators of HPAA activity. Unlike LPS, rhIL-6 was not pyrogenic in sheep at this challenge dose. In contrast, rhIL-6 elicited ACTH and cortisol responses that peaked earlier than those induced by LPS. These results suggest that this dose of IL-6, alone, is not sufficient to elicit the febrile response in sheep, however, it is a potent activator of the ovine HPAA response.
用重组人白细胞介素-6（rhIL-6；2.0微克/千克；n = 5）、大肠杆菌脂多糖（LPS）内毒素（400纳克/千克；n = 7）或生理盐水（n = 6）对绵羊进行免疫攻击，以确定IL-6是否激活绵羊的发热和下丘脑-垂体-肾上腺轴（HPAA）反应，并将这些反应与内毒素血症相关的反应进行比较。随着时间的推移采集血液，测量血浆促肾上腺皮质激素（ACTH）和血清皮质醇浓度，作为HPAA活性的指标。与LPS不同，在该攻击剂量下，rhIL-6在绵羊中不具有致热作用。相反，rhIL-6引起的ACTH和皮质醇反应峰值比LPS诱导的反应更早出现。这些结果表明，单独使用该剂量的IL-6不足以引发绵羊的发热反应，然而，它是绵羊HPAA反应的有效激活剂。
