Université d'Aix-Marseille I, Laboratoire de Neurobiologie Intégrative et Adaptative, UMR/CNRS 6149, 3 Place Victor Hugo, 13331 Marseille Cedex 03, France.
Neurobiol Learn Mem. 2010 Jul;94(1):42-56. doi: 10.1016/j.nlm.2010.03.005. Epub 2010 Mar 30.
The cholinergic neuronal system, through its projections to the hippocampus, plays an important role in learning and memory. The aim of the study was to identify genes and networks in rat hippocampus with and without memory deficit. Genome-scale screening was used to analyze gene expression changes in rats submitted or not to intraparenchymal injection of 192 IgG-saporin and trained in spatial/object novelty tasks. Results showed learning processes were associated with significant expression of genes that could be grouped into several clusters of similar expression profiles and that are involved in biological functions, namely lipid metabolism, signal transduction, protein metabolism and modification, and transcription regulation. Memory loss following hippocampal cholinergic deafferentation was associated with significant expression of genes that did not show similar cluster organization. Only one cluster of genes could be identified; it included genes that would be involved in tissue remodeling. More important, most of the genes significantly altered in lesioned rats were down-regulated.
胆碱能神经元系统通过其投射到海马体的作用，在学习和记忆中发挥着重要作用。本研究的目的是鉴定在有和没有记忆缺陷的大鼠海马体中与记忆相关的基因和网络。通过全基因组筛选，分析了接受或不接受脑内注射 192 IgG-saporin 并接受空间/物体新颖性任务训练的大鼠的基因表达变化。结果表明，学习过程与基因的显著表达有关，这些基因可以分为几个具有相似表达谱的聚类，并涉及生物功能，即脂质代谢、信号转导、蛋白质代谢和修饰以及转录调控。海马胆碱能去传入后的记忆丧失与基因的显著表达有关，这些基因没有表现出相似的聚类组织。只能确定一个基因簇；它包括参与组织重塑的基因。更重要的是，在受损大鼠中，大多数显著改变的基因都下调了。
