通过和频振动光谱研究由胆固醇和1,2-二硬脂酰-sn-甘油-3-磷酸胆碱组成的平面支撑脂质膜的相行为。
Phase Behavior of Planar Supported Lipid Membranes Composed of Cholesterol and 1,2-Distearoyl-sn-Glycerol-3-Phosphocholine Examined by Sum-Frequency Vibrational Spectroscopy.
作者信息
Liu Jin, Conboy John C
机构信息
University of Utah, Department of Chemistry, 315 S. 1400 E. RM 2020, Salt Lake City, Utah, 84112.
出版信息
Vib Spectrosc. 2009 May 26;50(1):106-115. doi: 10.1016/j.vibspec.2008.09.004.
The influence of cholesterol (CHO) on the phase behavior of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) planar supported lipid bilayers (PSLBs) was investigated by sum-frequency vibrational spectroscopy (SFVS). The intrinsic symmetry constraints of SFVS were exploited to measure the asymmetric distribution of phase segregated phospholipid domains in the proximal and distal layers of DSPC + CHO binary mixtures as a function of CHO content and temperature. The SFVS results suggest that cholesterol significantly affects the phase segregation and domain distribution in PSLBs of DSPC in a concentration dependent manner, similar to that found in bulk suspensions. The SFVS spectroscopic measurements of phase segregation and structure change in the binary mixture indicate that membrane asymmetry must be present in order for the changes in SFVS signal to be observed. These results therefore provide important evidence for the delocalization and segregation of different phase domain structures in PSLBs due to the interaction of cholesterol and phospholipids.
通过和频振动光谱（SFVS）研究了胆固醇（CHO）对1,2-二硬脂酰-sn-甘油-3-磷酸胆碱（DSPC）平面支撑脂质双层膜（PSLBs）相行为的影响。利用SFVS的固有对称性约束来测量DSPC + CHO二元混合物近端和远端层中相分离磷脂域的不对称分布，该分布是CHO含量和温度的函数。SFVS结果表明，胆固醇以浓度依赖的方式显著影响DSPC的PSLBs中的相分离和域分布，这与在本体悬浮液中发现的情况类似。二元混合物中相分离和结构变化的SFVS光谱测量表明，为了观察到SFVS信号的变化，膜必须存在不对称性。因此，这些结果为由于胆固醇和磷脂的相互作用导致PSLBs中不同相域结构的离域和分离提供了重要证据。
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