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本文引用的文献

1
Changes of the physical properties of the liquid-ordered phase with temperature in binary mixtures of DPPC with cholesterol: A H-NMR, FT-IR, DSC, and neutron scattering study.在 DPPC 与胆固醇的二元混合物中，随着温度的变化，液相有序相的物理性质的变化：H-NMR、FT-IR、DSC 和中子散射研究。
Biophys J. 1992 Apr;61(4):1025-35. doi: 10.1016/S0006-3495(92)81910-0.
2
Sum frequency generation from Langmuir-Blodgett multilayer films on metal and dielectric substrates.金属和电介质基底上朗缪尔－布洛杰特多层膜的和频产生
J Phys Chem B. 2005 Oct 13;109(40):18723-32. doi: 10.1021/jp051564y.
3
Thermodynamic equilibrium of domains in a two-component Langmuir monolayer.双组分朗缪尔单分子层中畴的热力学平衡
Biophys J. 2006 Jul 15;91(2):444-53. doi: 10.1529/biophysj.106.081000. Epub 2006 Apr 21.
4
Lateral phase separation in lipid-coated microbubbles.脂质包被微泡中的侧向相分离
Langmuir. 2006 Apr 25;22(9):4291-7. doi: 10.1021/la052841v.
5
The diversity of the liquid ordered (Lo) phase of phosphatidylcholine/cholesterol membranes: a variable temperature multinuclear solid-state NMR and x-ray diffraction study.磷脂酰胆碱/胆固醇膜的有序液相（Lo）相的多样性：变温多核固态核磁共振和X射线衍射研究
Biophys J. 2006 Apr 1;90(7):2383-93. doi: 10.1529/biophysj.104.056499.
6
The phase transition behavior of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) model membrane influenced by 2,4-dichlorophenol--an FT-Raman spectroscopy study.2,4-二氯苯酚对1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）模型膜相变行为的影响——傅里叶变换拉曼光谱研究
Chem Phys Lipids. 2006 Feb;139(2):115-24. doi: 10.1016/j.chemphyslip.2005.11.005. Epub 2006 Jan 18.
7
On the quantitative interpretation of biomembrane structure by Raman spectroscopy.关于通过拉曼光谱对生物膜结构进行定量解释
Biochim Biophys Acta. 1977 Mar 1;465(2):260-74. doi: 10.1016/0005-2736(77)90078-5.
8
Structure of a gel phase lipid bilayer prepared by the Langmuir-Blodgett/Langmuir-Schaefer method characterized by sum-frequency vibrational spectroscopy.通过和频振动光谱表征的采用朗缪尔-布洛杰特/朗缪尔-谢弗方法制备的凝胶相脂质双层的结构。
Langmuir. 2005 Sep 27;21(20):9091-7. doi: 10.1021/la051500e.
9
1,2-diacyl-phosphatidylcholine flip-flop measured directly by sum-frequency vibrational spectroscopy.通过和频振动光谱直接测量1,2 - 二酰基磷脂酰胆碱的翻转。
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10
Do you believe in lipid rafts? Biologists are turning to several analytical techniques to find out whether lipid rafts really exist?你相信脂筏的存在吗？生物学家正在采用多种分析技术来探究脂筏是否真的存在。
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通过和频振动光谱研究由胆固醇和1,2-二硬脂酰-sn-甘油-3-磷酸胆碱组成的平面支撑脂质膜的相行为。

Phase Behavior of Planar Supported Lipid Membranes Composed of Cholesterol and 1,2-Distearoyl-sn-Glycerol-3-Phosphocholine Examined by Sum-Frequency Vibrational Spectroscopy.

作者信息

Liu Jin, Conboy John C

机构信息

University of Utah, Department of Chemistry, 315 S. 1400 E. RM 2020, Salt Lake City, Utah, 84112.

出版信息

Vib Spectrosc. 2009 May 26;50(1):106-115. doi: 10.1016/j.vibspec.2008.09.004.

DOI:10.1016/j.vibspec.2008.09.004
PMID:20361007
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2846528/
Abstract

The influence of cholesterol (CHO) on the phase behavior of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) planar supported lipid bilayers (PSLBs) was investigated by sum-frequency vibrational spectroscopy (SFVS). The intrinsic symmetry constraints of SFVS were exploited to measure the asymmetric distribution of phase segregated phospholipid domains in the proximal and distal layers of DSPC + CHO binary mixtures as a function of CHO content and temperature. The SFVS results suggest that cholesterol significantly affects the phase segregation and domain distribution in PSLBs of DSPC in a concentration dependent manner, similar to that found in bulk suspensions. The SFVS spectroscopic measurements of phase segregation and structure change in the binary mixture indicate that membrane asymmetry must be present in order for the changes in SFVS signal to be observed. These results therefore provide important evidence for the delocalization and segregation of different phase domain structures in PSLBs due to the interaction of cholesterol and phospholipids.

摘要

通过和频振动光谱（SFVS）研究了胆固醇（CHO）对1,2-二硬脂酰-sn-甘油-3-磷酸胆碱（DSPC）平面支撑脂质双层膜（PSLBs）相行为的影响。利用SFVS的固有对称性约束来测量DSPC + CHO二元混合物近端和远端层中相分离磷脂域的不对称分布，该分布是CHO含量和温度的函数。SFVS结果表明，胆固醇以浓度依赖的方式显著影响DSPC的PSLBs中的相分离和域分布，这与在本体悬浮液中发现的情况类似。二元混合物中相分离和结构变化的SFVS光谱测量表明，为了观察到SFVS信号的变化，膜必须存在不对称性。因此，这些结果为由于胆固醇和磷脂的相互作用导致PSLBs中不同相域结构的离域和分离提供了重要证据。