人尿液中尿囊素的超高效液相色谱-串联质谱定量分析。
Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry.
机构信息
Department of Pediatrics, Duke University Medical Center, Durham NC 27709, USA.
出版信息
Anal Biochem. 2010 Jul 15;402(2):191-3. doi: 10.1016/j.ab.2010.03.033. Epub 2010 Mar 31.
Abstract
Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography-tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error=6%), precise (intra- and interday imprecision <8%), and sensitive (limit of detection=0.06pmol). Allantoin levels measured in control samples were comparable to literature values.
摘要
尿酸是一种有效的抗氧化剂和单线态氧及其他自由基的清除剂。别嘌醇，尿酸自由基诱导氧化的主要产物，在尿液中被有效排泄，具有作为氧化应激生物标志物的潜力。我们开发了一种使用超高效液相色谱-串联质谱的快速和特异的尿液别嘌醇检测方法，适合高通量的临床研究。该方法需要的样本制备量最少，并且准确（平均误差=6%）、精密（日内和日间精密度<8%）、灵敏（检测限=0.06pmol）。在对照样本中测量的别嘌醇水平与文献值相当。
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