活化白细胞细胞黏附分子（ALCAM/CD166）在原发性乳腺癌和远处乳腺癌转移中的表达水平。

Expression levels of Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166) in primary breast carcinoma and distant breast cancer metastases.

机构信息

Department of Gynecology, University Medical Center Hamburg-Eppendorf, Martinistr. 52, D-20246 Hamburg, Germany.

出版信息

Dis Markers. 2010;28(2):71-8. doi: 10.3233/DMA-2010-0685.

DOI:10.3233/DMA-2010-0685

PMID:20364042

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3833419/

Abstract

INTRODUCTION

Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166) gained increasing attention regarding tumorprogression and metastatic spread in breast cancer. The aim of this study was to examine ALCAM expression levels in primary breast cancer and distant metastases of the same patient within 29 autopsy cases to better understand the underlying mechanisms of metastases and the role of adhesion molecules in this process.

MATERIAL AND METHODS

Paraffin-embedded tissue of the primary and distant metastases (N=84) were collected and ALCAM immunohistochemistry was performed.

RESULTS

The primary tumor and all metastases showed a statistically normally distributed ALCAM expression. ALCAM expression level average differs between immunoreactive score (IRS) (mean) 4.16 (lung)-5.00 (adrenal gland). Of the metastatic ALCAM expression levels we obtained an intra-class correlation (ICC) of 80.9%, indicating a strong cluster effect of measurements in the same patient. ALCAM expression scores in metastatic sites and in the primary analyzed by hierarchical regression analysis showed that ALCAM expression in the primary is prognostic for ALCAM expression in all different sites of metastases (slope=0.773, p < 0.001, r(2)= 0.504).

CONCLUSION

ALCAM expression in the primary is positively correlated to ALCAM expression in metastases within one single patient. This could show a tumorbiological context of ALCAM for the development of metastases in breast cancer.

摘要

简介

活化白细胞细胞黏附分子（ALCAM/CD166）在乳腺癌的肿瘤进展和转移扩散方面受到越来越多的关注。本研究的目的是在 29 例尸检病例中检测原发性乳腺癌和同一患者的远处转移中 ALCAM 的表达水平，以更好地了解转移的潜在机制以及黏附分子在该过程中的作用。

材料与方法

收集原发性和远处转移的石蜡包埋组织（N=84），并进行 ALCAM 免疫组织化学染色。

结果

原发性肿瘤和所有转移灶的 ALCAM 表达均呈统计学正态分布。免疫反应评分（IRS）（平均值）4.16（肺）-5.00（肾上腺）之间的 ALCAM 表达水平存在差异。我们获得了转移性 ALCAM 表达水平的组内相关系数（ICC）为 80.9%，表明同一患者的测量具有很强的聚类效应。通过分层回归分析对转移性部位和原发性的 ALCAM 表达评分进行分析表明，原发性的 ALCAM 表达与所有不同转移部位的 ALCAM 表达呈正相关（斜率=0.773，p <0.001，r²=0.504）。