从大鼠HTC-H1细胞与K-NRK细胞的共培养物中分离出的一种转化病毒KiMSV（RHHV）的特性。

Properties of a transforming virus, KiMSV(RHHV), isolated from a co-culture of rat HTC-H1 cells with K-NRK cells.

作者信息

Yang S S, Malech H L, Wu R S, Woronow D I

出版信息

J Gen Virol. 1978 Feb;38(2):209-21. doi: 10.1099/0022-1317-38-2-209.

PMID:203653

Abstract

Some morphological, biological, immunological and biochemical characterizations of a virus, rat helper virus pseudotype Kirsten sarcoma virus, KiMSV(RHHV), have been presented here. KiMSV(RHHV) has a type C virus ultrastructure. It is strictly rat tropic and is able to transform rat cells in vitro. The possibility of its being a xenotropic mouse virus has been carefully ruled out by exhaustive analyses of host range and immunological studies. Antigenically KiMSV(RHHV) demonstrates cross reactivity with an antiserum specific against rat leukaemia virus, no cross reactivity with antiserum against Moloney leukaemia virus, and only minor cross reactivity with antiserum against cat leukaemia virus. Analysis of virus proteins and glycoproteins by equilibrium acrylamide gradient gel electrophoresis showed that the virus complex possesses both a gp70 fraction and a p30 fraction. KiMSV(RHHV) sediments isopycnically in a linear sucrose gradient at 1.145 to 1.155 g/ml and possesses RNA and reverse transcriptase activity.

摘要

本文介绍了一种病毒——大鼠辅助病毒假型克氏肉瘤病毒（KiMSV，RHHV）的一些形态学、生物学、免疫学和生物化学特征。KiMSV（RHHV）具有C型病毒的超微结构。它严格嗜大鼠，能够在体外转化大鼠细胞。通过对宿主范围的详尽分析和免疫学研究，已仔细排除了它是嗜异性小鼠病毒的可能性。在抗原性方面，KiMSV（RHHV）与抗大鼠白血病病毒的抗血清表现出交叉反应，与抗莫洛尼白血病病毒的抗血清无交叉反应，与抗猫白血病病毒的抗血清仅有轻微交叉反应。通过平衡丙烯酰胺梯度凝胶电泳对病毒蛋白和糖蛋白进行分析表明，病毒复合物同时具有gp70组分和p30组分。KiMSV（RHHV）在1.145至1.155 g/ml的线性蔗糖梯度中以等密度方式沉降，并具有RNA和逆转录酶活性。