Decker B, Bosch U, Kasperczyk W, Oestern H J, Reale E
Laboratory of Cell Biology and Electron Microscopy, Hannover Medical School, FRG.
J Submicrosc Cytol Pathol. 1991 Jan;23(1):9-21.
In four black-faced sheep, the posterior cruciate ligament was replaced with a free autogenous patellar tendon transplant. Tissue samples from the transplants were investigated by light and electron microscopy 1 year and 2 years after surgery. The normal contralateral posterior cruciate ligament and the normal contralateral patellar tendon were used as controls. The structural differences concerned cells, collagen fibrils, elastic tissue and proteoglycans. Most of the cells of the contralateral patellar tendon were spindle-shaped, whereas those of the transplant were frequently chondroid. In the central region of the transplant as well as in the area far from the bone, cell degenerations, and occasionally hypo- or even acellular zones were found. Measurements of the diameter of collagen fibrils in both contralateral patellar tendon and posterior cruciate ligament showed a more or less pronounced bimodal distribution. A unimodal distribution with mainly thin fibrils (20-60 nm) was demonstrated in the transplant tissue which also revealed some morphological alterations of the collagen fibrils. Thin elastic fibers (microfibrils and amorphous material) were randomly scattered among the collagen fibrils of the control samples, bundles of microfibrils (without amorphous material) characterized the transplant. Staining with Alcian blue in the presence of 0.3 M MgCl2 demonstrated a close relationship between proteoglycans and collagen fibrils as well as elastic components in patellar tendon. This arrangement was lost in the transplant where abundant proteoglycans were revealed which, however, composed a tight irregular network between the collagen fibrils. The results serve as a baseline for understanding the impaired biochemical properties of a free autogenous patellar tendon transplant.
在四只黑脸绵羊中，用自体游离髌腱移植替代后交叉韧带。术后1年和2年，通过光镜和电镜对移植组织样本进行研究。将对侧正常后交叉韧带和对侧正常髌腱用作对照。结构差异涉及细胞、胶原纤维、弹性组织和蛋白聚糖。对侧髌腱的大多数细胞呈纺锤形，而移植组织中的细胞常呈软骨样。在移植组织的中央区域以及远离骨骼的区域，发现了细胞退变，偶尔还有细胞减少甚至无细胞区。对侧髌腱和后交叉韧带中胶原纤维直径的测量显示出或多或少明显的双峰分布。移植组织中呈现出主要为细纤维（20 - 60纳米）的单峰分布，且胶原纤维也显示出一些形态学改变。细弹性纤维（微原纤维和无定形物质）随机散布在对照样本的胶原纤维之间，而移植组织的特征是微原纤维束（无无定形物质）。在0.3M氯化镁存在下用阿尔辛蓝染色显示，髌腱中的蛋白聚糖与胶原纤维以及弹性成分之间存在密切关系。在移植组织中这种排列消失，其中显示出大量蛋白聚糖，然而，它们在胶原纤维之间构成了紧密的不规则网络。这些结果为理解自体游离髌腱移植受损的生化特性提供了基线。
