Hurlburt E M, Ki P F, Wagner H N
J Nucl Med. 1978 Feb;19(2):191-4.
The effect of cytomegalovirus on the metabolism in monolayers of human embryonic lung fibroblasts (WI-38 cells) was studied. Effects of viral infection were examined by comparing [3H] thymidine incorporation in infected cells with that in uninfected cells. The time for detecting changes in cellular metabolism using the radiometric method was compared with that for observing cytopathic effects in infected cells. Compared with uninfected cells, cells infected with 10(4) TCID50 of virus showed nearly 400% increased in [3H] thymidine uptake 48 hr after infection. The radiometric method was able to detect 10(3) TCID50 of virus, with about 250% stimulation, 24--48 hr before visible signs of cytopathic effects. Our results suggest that with further development, radiometric measurement of metabolism in cytomegalovirus-infected cell cultures might provide a means of detecting viral presence in clinical assays. The radiometric method has the advantage of objectivity and potential for automation.
研究了巨细胞病毒对人胚肺成纤维细胞（WI - 38细胞）单层代谢的影响。通过比较感染细胞与未感染细胞中[³H]胸苷掺入情况来检测病毒感染的影响。将使用放射测量法检测细胞代谢变化的时间与观察感染细胞中细胞病变效应的时间进行了比较。与未感染细胞相比，感染10⁴半数组织培养感染剂量（TCID₅₀）病毒的细胞在感染后48小时[³H]胸苷摄取量增加了近400%。放射测量法能够在出现细胞病变效应可见迹象前24 - 48小时检测到10³TCID₅₀的病毒，刺激约250%。我们的结果表明，随着进一步发展，对巨细胞病毒感染的细胞培养物进行代谢的放射测量可能为临床检测病毒存在提供一种方法。放射测量法具有客观性和自动化潜力的优点。
