先兆子痫患者母血和脐血中纤连蛋白浓度升高与胎盘透明质酸和羟脯氨酸含量增加有关。

Increased maternal serum and cord blood fibronectin concentrations in preeclampsia are associated with higher placental hyaluronic acid and hydroxyproline content.

作者信息

Uzun Hafize, Konukoglu Dildar, Albayrak Mustafa, Benian Ali, Madazli Riza, Aydin Seval, Gelisgen Remisa, Uludag S

机构信息

Istanbul University, Cerrahpaşa Medical Faculty, Department of Biochemistry, Istanbul, Turkey.

出版信息

Hypertens Pregnancy. 2010 Jan;29(2):153-62. doi: 10.3109/10641950902968619.

DOI:10.3109/10641950902968619

PMID:20367505

Abstract

BACKGROUND

Total or cellular fibronectin (FN) determinations have been used to differentiate between normal and preeclamptic pregnants. The purpose of this study was to examine the relationship between maternal serum FN levels and the extracellular matrix molecule contents of placental tissue, such as FN, hyaluronic acid (HA) and hydroxyproline (HP) levels.

MATERIAL AND METHODS

We obtained maternal blood samples and placental tissue samples from healthy (n = 17, controls) and preeclamptic pregnants (n = 29). We also obtained cord blood samples for FN and HA determination from the same patients. FN and HA concentrations in the placenta and maternal and cord blood were measured by and enzyme-linked immunosorbent assay and HP contents in the placenta were measured by a colorimetric assay.

RESULTS

FN levels in maternal serum, cord blood, and placenta were significantly higher in preeclamptics than in controls (p<0.001, p<0.001 and p<0.05, respectively). HA concentrations in the cord blood and placenta were found to be elevated in preeclamptics (p<0.05 and p<0.01). Preeclamptics had significantly higher placental HP levels than controls (p<0.001). Similar statistically significant results were obtained when the pregnant subjects classified as nulliparous and multiparous. There was no difference in ECM molecule levels between nulliporous and multiparous women in preeclamptic pregnant group. In regression analysis maternal serum FN levels were correlated with placental HA and HP levels (p<0.01 and p<0.01). There was a positive correlation between cord blood FN and both placental HP (p<001) and HA levels (p<0.01). FN levels in maternal serum, cord blood, and placenta were also negative correlated with fetal birth weight (p<0.01, p<0.05 and p<0.05, respectively).

CONCLUSION

FN in maternal serum, cord blood, and placenta is increased with elevated placental HA and HP levels, probably reflecting placental basement membrane alterations during preeclampsia.

摘要

背景

总纤连蛋白（FN）或细胞纤连蛋白测定已用于区分正常孕妇和先兆子痫孕妇。本研究的目的是探讨母体血清FN水平与胎盘组织细胞外基质分子含量之间的关系，如FN、透明质酸（HA）和羟脯氨酸（HP）水平。

材料与方法

我们从健康孕妇（n = 17，对照组）和先兆子痫孕妇（n = 29）中获取母体血液样本和胎盘组织样本。我们还从同一患者中获取脐带血样本用于FN和HA测定。通过酶联免疫吸附测定法测量胎盘、母体和脐带血中的FN和HA浓度，并通过比色法测量胎盘中的HP含量。

结果

先兆子痫孕妇的母体血清、脐带血和胎盘中的FN水平显著高于对照组（分别为p<0.001、p<0.001和p<0.05）。发现先兆子痫孕妇脐带血和胎盘中的HA浓度升高（p<0.05和p<0.01）。先兆子痫孕妇的胎盘HP水平显著高于对照组（p<0.001）。当将怀孕受试者分为初产妇和经产妇时，也获得了类似的统计学显著结果。先兆子痫孕妇组中初产妇和经产妇之间的细胞外基质分子水平没有差异。在回归分析中，母体血清FN水平与胎盘HA和HP水平相关（p<0.01和p<0.01）。脐带血FN与胎盘HP（p<0.01）和HA水平（p<0.01）均呈正相关。母体血清、脐带血和胎盘中的FN水平也与胎儿出生体重呈负相关（分别为p<0.01、p<0.05和p<0.05）。