[Gene polymorphism of tumor necrosis factor-alpha receptor II in 196 site and premature births in Chinese Han Population].

OBJECTIVE

To investigate the relationship between tumor necrosis factor receptor II (TNFR II) gene 196T/G polymorphism and preterm labor in Han population in Chengdu.

METHODS

Samples were collected from 96 subjects in corresponding period, 46 preterm labor pregnant women (we collected partial placental tissues of preterm labor, 21 cases of infectious group with chorioamnionitis, 25 cases of noninfectious group without chorioamnionitis), and 50 normal labor pregnant women. The DNA was extracted from each sample by using Chelex-100 method, then PCR-RFLP was performed to determine the TNFR II 196 gene polymorphism.

RESULTS

1. TNFR II 196 genotype frequencies of 196M/M (TT), 196M/R (TG) + 196R/R (GG) were 71.7%, 21.7%+ 6.5% and 80.0%, 20.0%+0.0% in preterm labor and normal control group respectively. Allele frequencies of R (G), M (T) were 17.4%, 82.6% and 10.0%, 90.0%, respectively. There were no significant difference in frequencies of genotype and allele in TNFR II 196 gene polymorphism between two groups (P > 0.05, P > 0.05, respectively). 2) Close correlation was observed between the different genotypes and the chorioamnionitis (chi2 = 11.088, P < 0.05). The odds ratio (OR) for TG+GG genotype was 12.65, 95% CI 2.359, 67.848, with more than 12.65 times probability of chorioamnionitis than that of TT genotype group.

CONCLUSION

Polymorphism in 196 site of TNFR II gene was not crucial in preterm labor genesis, TG (GG) genotype may contribute to susceptibility to chorioamnionitis in the process of preterm labor in Chinese Han population.