使用荧光光激活技术对神经元细胞骨架动态进行活细胞成像。

Live cell imaging of cytoskeletal dynamics in neurons using fluorescence photoactivation.

机构信息

Department of Neurobiology, University of Osnabrück, Barbarastrasse 11, D-49076 Osnabrück, Germany.

出版信息

Biol Chem. 2010 Jun;391(6):639-43. doi: 10.1515/BC.2010.060.

DOI:10.1515/BC.2010.060

PMID:20370315

Abstract

Alzheimer's disease and other neurodegenerative disorders share some common features at the cellular level, which are often associated with a change in cytoskeletal dynamics. Live cell imaging has been applied to study various aspects of cell physiology including cytoskeletal dynamics. Recently, fluorescence photoactivation (FPA) has been developed as a novel approach to analyze protein dynamics in living cells with high sensitivity. Here, we describe the application of FPA to determine interactions and mobility of the microtubule-associated protein tau in neurons as an example for a disease-related cytoskeletal protein and discuss further applications of FPA to study cytoskeletal dynamics in neurodegenerative diseases.

摘要

阿尔茨海默病和其他神经退行性疾病在细胞水平上具有一些共同特征，这些特征通常与细胞骨架动力学的变化有关。活细胞成像已被应用于研究细胞生理学的各个方面，包括细胞骨架动力学。最近，荧光光激活（FPA）已被开发为一种新的方法，用于以高灵敏度分析活细胞中的蛋白质动力学。在这里，我们以微管相关蛋白 tau 为例，描述了 FPA 在神经元中确定相互作用和流动性的应用，作为与疾病相关的细胞骨架蛋白的一个例子，并讨论了 FPA 在神经退行性疾病中研究细胞骨架动力学的进一步应用。