Mills R G, Atkins A R, Harvey T, Junius F K, Smith R, King G F
Department of Biochemistry, University of Sydney, NSW, Australia.
FEBS Lett. 1991 May 6;282(2):247-52. doi: 10.1016/0014-5793(91)80488-o.
The solution structure of sarafotoxin-6b in water has been determined using high-resolution NMR spectroscopy. 127 proton-proton distance measurements and three phi dihedral angle constraints derived from NMR spectra were used to calculate the solution structure using a combination of distance geometry and restrained molecular dynamics. The major structural feature of the resulting family of five structures was a right-handed alpha-helix extending from K9 to Q17. In contrast, the C-terminal region of the peptide appears not to adopt a preferred conformation in aqueous solution. The present structure is compared with those previously determined for endothelin peptides in non-aqueous solvents.
