Purification and partial characterization of glyoxalase I from a higher plant Brassica juncea.
作者信息
Deswal R, Sopory S K
机构信息
Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
出版信息
FEBS Lett. 1991 May 6;282(2):277-80. doi: 10.1016/0014-5793(91)80494-n.
PMID:2037046
Abstract
The glyoxalase I was purified from Brassica juncea by affinity chromatography on S-hexyl GSH sepharose 4B. Homogeneity of the protein was confirmed electrophoretically by a silver stained gel. Activity staining on a native starch gel also showed a single band. The effect of glutathione, methylglyoxal, and pH on enzyme kinetics was studied. Magnesium was found to stimulate the enzyme activity.
摘要
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